HPLC测定半夏厚朴汤不同煎液中厚朴酚  

Determination of Magnolol in Different Decoctions of Banxia Houputang by HPLC

作　　者：冯华[1] 王祥培[2] 聂明华[1] 罗秀琼[1] 邹孔强 靳凤云[2] 

机构地区：[1]遵义市药品检验所,贵州遵义563002 [2]贵阳中医学院,贵阳550002

出　　处：《中国实验方剂学杂志》2011年第10期82-84,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：遵义市中药现代化科技产业研究开发专项项目课题[(2009)4号]

摘　　要：目的:考察用合煎、分煎方法制备的半夏厚朴汤汤剂中厚朴酚含量的变化。方法:建立高效液相色谱方法,采用Diamonisl C18柱(4.6 mm×200 mm,5μm);流动相为甲醇-水(78∶22),流速1 mL.min-1,检测波长为294 nm。分析半夏厚朴汤合煎液和分煎液样品中厚朴酚的含量变化。结果:半夏厚朴汤分煎液中厚朴酚的平均含量等于合煎液中厚朴酚的平均含量。结论:分煎液与合煎液比较,2种煎液中厚朴酚的含量无差异性。Objective：To investigate the content of magnolol in hejian decoction（mixed the traditional Chinese herbs together,then boiling them with water）and in fenjian decoction（boiling the single traditional Chinese herb with water separately,then mixed the Abstracts）of Banxia Houputang（composed of Prunus Ephedra sinica armeniaca and Mangnolia officinalis）.Method：A HPLC method was established with a Diamonisl C18 column（4.6 mm×200 mm,5 μm）,a mobile phase of methanol-water（78∶22）,a flow rate of 1 mL·min-1 and a detection wavelength of 294 nm in order to determine the content of magnolol.Result：The average content of magnolol of the fenjian decoction was equal to that of the hejian decoction.Conclusion：Comparing with both decoctions,the content of magnolol in hejian and fenjian decoction was equal.

关 键 词：半夏厚朴汤 厚朴酚 合煎 分煎 高效液相谱法 

分 类 号：R284.1[医药卫生—中药学]