瓜蒌薤白汤对肺纤维化形成阶段的干预作用  

Intervention of Gualou Xiebai Decoction on Pulmonary Fibrosis Formation Stage

作　　者：宋建平[1] 谢忠礼[1] 李伟[1] 李瑞琴[1] 张瑞[1] 杨美凤[1] 

机构地区：[1]河南中医学院,郑州450008

出　　处：《中国实验方剂学杂志》2011年第10期140-143,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省科技攻关项目(0424410103)

摘　　要：目的:观察瓜蒌薤白汤对大鼠肺纤维化形成阶段(造模后14～28 d)肺部病理损害和肺组织去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)含量的影响。方法:普通级雌性Wistar大鼠随机分为正常组、模型组、泼尼松组、瓜蒌薤白汤组。除正常组外,其余各组均采用气管内一次性注入平阳霉素(0.003 g·kg-1体重)复制肺纤维化模型,造模14 d后,泼尼松组以泼尼松0.004 g·kg-1体重ig,瓜蒌薤白汤组以全瓜蒌2.04 g·kg-1体重,薤白1.02 g·kg-1体重ig,第28天处死动物,比较各组肺指数、肺纤维化程度、肺组织DA,NE,5-HT含量。结果:模型组肺纤维化程度重于正常组、泼尼松组与瓜蒌薤白汤组(P<0.05),肺指数高于正常组与各用药组(P<0.05),肺组织DA,NE,5-HT含量高于正常组、泼尼松组(P<0.05);DA:模型组(60.57±8.47)ng·g-1,正常组(20.59±6.68)ng·g-1,泼尼松组(18.44±4.16)ng·g-1;NE:模型组(37.01±4.99)ng·g-1,正常组(26.91±1.90)ng·g-1,泼尼松组(23.06±5.64)ng·g-1;5-HT:模型组(88.87±5.02)ng·g-1,正常组(20.31±4.24)ng·g-1,泼尼松组(69.18±9.00)ng·g-1;瓜蒌薤白汤组NE含量(46.31±4.55)ng·g-1明显高于模型组,DA(27.48±3.20)ng·g-1,5-HT(48.90±8.47)ng·g-1含量低于模型组(P<0.05)。结论:瓜蒌薤白汤具有减轻肺纤维化模型形成阶段肺病理改变的作用;肺纤维化形成阶段肺中DA,NE,5-HT升高;瓜蒌薤白汤能抑制模型肺DA,5-HT含量的升高,增加模型肺中NE含量。Objective：To observe the effect of Gualou Xiebai decoction to pathological lesion and dopamine（DA）,norepinephrine（NE）,5-hydroxytryptamine（5-HT）in lung at stage of pulmonary fibrosis formation in rats.Method：The female Wistar rats were divided into control,model,prednisone and Gualou Xiebai decoction groups randomly.The model was induced by injected pingyangmycin in trachea only once.The drugs were used after 14 days as treatments.After 28 days of the model establishment,the rats were sacrificed.The lung index,pulmonary fibrosis degree,contents of DA,NE and 5-HT in lung tissue were evaluated and compared.Result：The pulmonary fibrosis degree was obviously severe in model than other groups（P0.05）and the lung index was high in the model than other groups（P0.05）.The contents of DA,NE and 5-HT in lung tissue were obviously high in the model compared with the control and prednisone groups（P0.05）.The contents of NE were obviously high than that in model group,but contents of DA and 5-HT were lower in Gualou Xiebai decoction than model group（P0.05）.Conclusion：Gualou Xiebai decoction had the function to lessen lung pathology change in pulmonary fibrosis formation stage.The DA,NE and 5-HT were high in the pulmonary fibrosis formation stage.Gualou Xiebai decoction had the function to restrain the increase in DA and 5-HT and raise the NE content in model lung.

关 键 词：肺纤维化 瓜蒌薤白汤 去甲肾上腺素 多巴胺 5-羟色胺 

分 类 号：R285.5[医药卫生—中药学]