补肾活血汤对制动应激雌性大鼠生殖内分泌功能紊乱的影响  

Effect of Bushen Huoxuetang on Reproductive-endocrine Disorder Induced by Immobilization Stress in Femail Rats

作　　者：李兰英[1] 王佩娟[1] 彭蕴茹[1] 罗宇慧[1] 韩关绥[1] 

机构地区：[1]江苏省中医药研究院,江苏省中西医结合医院,南京210028

出　　处：《中国实验方剂学杂志》2011年第10期144-147,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省自然科学基金项目(BK2007174);康缘中医药科技创新基金项目(HZ0811KY)

摘　　要：目的:探讨补肾活血汤对制动应激所致大鼠生殖内分泌功能紊乱的影响。方法:采用重复制动应激法制造大鼠生殖内分泌功能紊乱模型,按8.0,4.0,2.0 g·kg-1分别ig给予补肾活血汤,以放免法检测大鼠血清中性激素:雌二醇(E2)、孕酮(P)、黄体生成素(LH)、卵泡刺激素(FSH)水平;酶免法检测血清中激活素(ACTA)、抑制素(INHB)、卵泡抑素(FS)水平。结果:补肾活血汤8.0,4.0 g·kg-1剂量组可明显升高模型大鼠血清中E2水平,降低异常升高的ACTA水平,升高INHB和FS水平(P<0.05或P<0.01),对生殖内分泌功能紊乱大鼠的卵巢功能有明显的保护作用。结论:补肾活血汤对制动应激所致大鼠生殖内分泌功能紊乱的卵巢功能有明显的保护作用。Objective：To study the effects of Bushen Huoxuetang（BSHXT）in femail rats with Reproductive-endocrine Disorder（RED）induced by immobilization stress.Method：The female rat model of RED was established by repeated immobilization stress.The serum levels of estradiol（E2）,progesterone（P）,follicle stimulating hormone（FSH）and luteiuizing hormone（LH）were measured by radioimmunoassay（RIA）and,inhibin-B（INHB）,follistatin（FS）and activin-a（ACTA）were measured by enzyme-linked immunosorbent assay（ELISA）.Result：The serum level of E2 was significantly increased and the serum levels of INHB,FS and ACTA were obviously reduced in femail rat model treated by larger and middle doses of BSHXT（8.0,4.0 g·kg-1）.Conclusion：BSHXT could obviously protect ovarian function of RED induced by immobilization stress in femail rats.

关 键 词：补肾活血汤 制动应激 生殖内分泌功能紊乱 激素及分泌因子 

分 类 号：R285.5[医药卫生—中药学]