混合血小板凝胶促进新西兰兔创面愈合的实验研究  

The evaluation of pooled human platelet gel in the treatment of wound of New-Zealand rabbit

作　　者：李艳辉[1,2] 单桂秋[1] 张雅妮[1] 刘广亚[1,2] 吕品[1] 周谋[1] 丘勇新[1] 

机构地区：[1]广州军区广州总医院输血科,广东广州510010 [2]广州中医药大学

出　　处：《中国输血杂志》2011年第4期275-278,共4页Chinese Journal of Blood Transfusion

基　　金：广东省科技计划项目(编号:2009B080701092);广州市科技计划项目(10C36091671);国家科技重大专项--新药创制

摘　　要：目的研究人源性混合血小板凝胶对新西兰兔创面愈合的影响。方法采用手工分离的人源性混合血小板为原材料,加入不同激活剂(含有1 000 U/ml、100 U/ml、0 U/ml凝血酶的10%葡萄糖酸钙溶液)制备PG,血小板与激活剂按体积10∶1混合。新西兰兔24只雌雄各半随机分为4组,6只/组,每只制备2个创面,创面采用不同的敷料外敷:对照组敷料为无菌纱布,另3组(实验组)敷料为不同浓度凝血酶的激活剂制备的PG纱布。在术后d5、10、15、20更换敷料并切取病理组织标本,HE染色观察。结果实验组与对照组在创面愈合率的差异上无统计学意义(P>0.05),但肉眼观察创面愈合质量有较明显差异。组织学观察显示,实验组成纤维细胞排列整齐,紧密有序,细胞增生较快,新生血管出现早且丰富,新生皮肤质量明显优于对照组。结论人源性混合血小板凝胶能促进新西兰兔创面愈合,有临床研究和应用价值。Objective To investigate the effect of the pooled humen platelet gel on wound healing of New-Zealand rabbit.Methods Pooled human platelet-rich plasma and activating agents withwhich had different concentration of thrombin（10% calcium gluconate containing 1 000U/ml、100U/ml、0U/ml thrombin） were mixed in proportion of 10∶1 by the volume rates to get platelet gel（PG）.A total of 24 New Zealand rabbits（12 female and 12 male） a half were selected and randomly divided into four groups.Every group contain 6 New Zealand rabbits and two skin wounds were made to each rabbit had been created two skin defects.The skin defectwounds were treated with different dressing：the control group was with asepticsis gauze,the three experimental groups were with PG dressing prepared according to the methods above.OnAt days 5,10,15,and 20 after operation,the dressing of every defects were changed and tissue specimen of each group were cutted and investigatedstudied by histological observation.Results Although the difference inon wound healing rates of the control and experimental groups hadwere not statistical significant（P0.05）,the healing quality of the three experimental groups were obviously better than the control group by gross examination.Histological observation showed that the neogenesis skin of the three experimental groups was betterhave an advantage to than the control group：fibroblasts lined up in orderly and tightly,cells proliferated faster,new vessels appeared earlier and in larger quantitymore plentiful.Conclusion Pooled humaen platelet gel can promote the wound healing of New-Zealand rabbit and it has the potential to be applicateed to clinical research.

关 键 词：混合血小板 血小板凝胶 创面愈合 动物实验 

分 类 号：R641[医药卫生—外科学] R331.143[医药卫生—临床医学]