聚焦超声辐照兔左侧足三里穴后双耳痛耐受阈的变化研究  

A Study of Change of Pain Tolerance Thresholds of Bilateral Ears of Rabbit After Irradiation in Left Zusanli by Focused Ultrasound

作　　者：赵骏[1] 林强[1] 刘琳[1] 向理科[1] 邹建中[1] 白晋[1] 

机构地区：[1]重庆医科大学生物医学工程系,省部共建超声医学工程国家重点实验室,重庆400016

出　　处：《中国医科大学学报》2011年第5期390-393,共4页Journal of China Medical University

基　　金：国家中医药管理局资助项目(2007ZX01-2)

摘　　要：目的通过观察聚焦超声辐照兔左侧足三里穴后双耳痛耐受阈变化,探讨聚焦超声辐照足三里穴镇痛的可行性。方法 30只健康新西兰兔随机平均分为A、B 2组。A组左侧足三里穴以2W聚焦超声辐照5 min,B组同部位以无超声输出的假照5min。辐照前测双耳外侧近耳缘静脉处固定点痛耐受阈。辐照后1至16 min16个时间点,奇数时间点测右耳痛耐受阈,偶数时间点测左耳痛耐受阈,测量处同辐照前。于双侧足三里穴区取皮肤、肌肉组织进行HE染色观察。结果 A组辐照后,1 min至11min 6个时间点右耳痛耐受阈有显著提高(P<0.05),13 min、15 min痛耐受阈无明显变化(P>0.05),2 min至12 min 6个时间点左耳痛耐受阈有显著提高(P<0.05),14 min、16 min痛耐受阈无显著变化(P>0.05);辐照后1 min、3 min、5 min、7 min A组右耳痛耐受阈较B组有显著提高(P<0.05),4 min、6 min、8 min A组左耳痛耐受阈较B组有显著提高(P<0.05);B组辐照后双耳痛耐受阈无显著变化(P>0.05)。A组辐照后有精神不振现象,B组无此现象。辐照处及假照处穴区皮肤、肌肉组织较正常组织无明显变化。结论聚焦超声辐照兔左侧足三里穴可提高双耳痛耐受阈并维持一段时间,提示应用聚焦超声辐照穴位有镇痛效果且安全。Objective To explore the feasibility of focused ultrasound for analgesia by observing the change of pain tolerance thresholds of bilateral ears of each rabbit after a single irradiation by focused ultrasound in ＂Zusanli＂（ST36）.Methods Thirty healthy Zelanian rabbits irrespective of gender were selected and randomized divided into 2 groups（n =15）.The left ＂Zusanli＂（ST36） of each rabbit of group A was irradiated for 5 minutes by focused ultrasound with the power of 2 watts.The left ＂Zusanli＂（ST36） of each rabbit of group B was irradiated for 5 minutes without output of focused ultrasound.Pain tolerance threshold of fixed point near the edge of ear vein of each rabbit was measured before irradiation.Taking 16 time points after irradiation from the 1st min to the 16th min,pain tolerance threshold of right ear of each rabbit was measured at the odd time points,and the left＇s of each rabbit was measured at the even time points.Tissues of skins and muscles of bilateral ＂Zusanli＂（ST36） were taken,biopsy was performed with HE dyeing.Results Pain tolerance thresholds of right ears of rabbits of group A were enhanced observably in the 1st min to the 11th minute after irradiation（P〈0.05）,and in the 13th min and 15th min they were not different from those of before irradiation（P〉0.05）.The lefts＇ were enhanced observably in the 2nd min to the 12th minute after irradiation（P〈0.05）,and in the 13th min and 15th min they were no different from those of before irradiation（P〈0.05）.Pain tolerance thresholds of right ears of rabbits of group A were enhanced observably than those of group B after irradiation in the 1st min,3rd min,5th min and 7th min（P〈0.05）.The lefts＇ of rabbits of group A were enhanced observably than those of group B after irradiation in the 4th min,6th min,and 8th min（P〈0.05）.Pain tolerance thresholds of bilateral ears of rabbits of group B were not changed obviously after irradiation（P〉0.05）.Rabbits of group A were in low spirits a

关 键 词：聚焦超声 痛耐受阈 足三里 

分 类 号：R332[医药卫生—人体生理学]