高糖高脂诱导的H9c2细胞活性氧产物和线粒体功能障碍的激光扫描共聚焦显微镜观察  

Imaging of reactive oxygen species production and mitochondria dysfunction of high glucose and palmitate induced H9c2 cells by confocal laser scanning microscopy

作　　者：林敏[1] 李素菡[1] 杨静莹[1] 周武雄[1] 

机构地区：[1]上海中医药大学科技实验中心电镜室,201203

出　　处：《医学信息（下旬刊）》2011年第2期352-353,共2页Medical information

基　　金：基金项目：上海市科委浦江人才计划项目（06PJ14085）和2009年上海中医药大学中西医结合基础学科校级课题资助.

摘　　要：高血糖和高脂肪酸对细胞的功能有损害作用，即糖毒性和脂毒性。本文应用激光扫描共聚焦显微镜技术观察了培养的大鼠心肌源性H9c2细胞在高葡萄糖和棕榈酸的共同作用下细胞内活性氧的产生和线粒体膜电位的去极化，结果表明，5．5mmol／L葡萄糖和0．25mmol／L棕榈酸的共同作用能引起H9c2细胞内活性氧的产生和和线粒体膜电位的去极化，提示活性氧的积聚与细胞线粒体膜电位的丧失相关。High glucose and free fatty acids have been suggested to have-adverse effects on cell function, so called glueotoxicity and lipotoxicity. Glucose and palmitie acid induce oxidative stress and dysfunction of mitochondria in H9c2 cells was observed by confocal laser scanning microscopy. Intracellular reactive oxygen species level was determined by using the oxidant--sensitive fluorogenic probe CM--H2DCFDA. Mitochondria function was assayed by MitoTracker Orange CMTMRos. There was an increased production of reactive oxygen species and loss of mitochondria membrane potential after treatment with glucose and palmitate.

关 键 词：激光扫描共聚焦显微镜 活性氧 线粒体膜电位 

分 类 号：R589[医药卫生—内分泌]