大鼠骨髓间充质干细胞对T细胞免疫活性的影响  

Immunoregulatory effects of rat bone marrow mesenchymal stem cells on T cells

作　　者：单保恩[1] 杨兴肖[1] 马鸣[2] 刘丽华[1] 艾军[1] 赵连梅[1] 张小芳[1] 

机构地区：[1]河北医科大学第四医院科研中心,石家庄050011 [2]河北医科大学第四医院检验科,石家庄050011

出　　处：《中国免疫学杂志》2011年第5期409-413,共5页Chinese Journal of Immunology

基　　金：河北省高校强势特色学科(No.200552)资助项目

摘　　要：目的:探讨大鼠骨髓间充质干细胞(Mesenchymal stemcells,MSCs)对脾脏T细胞的免疫调节作用。方法:从大鼠骨髓中分离培养间充质干细胞,通过瑞氏-姬姆萨染色观察细胞形态,并用流式细胞术(Flowcytometry,FCM)检测其细胞表面特征分子表达情况。MTT法测定经刀豆蛋白A(ConA)刺激后,不同数量MSCs对T细胞增殖能力的影响;ELISA法检测MSCs对T细胞分泌IFN-γ和IL-4水平的影响。FCM检测MSCs对脾脏T细胞凋亡水平的影响。MTT法测定MSCs对细胞毒性T细胞(Cytotoxic Tcells,CTL)杀伤活性的影响。结果:经不同数量MSCs作用后,脾脏T细胞增殖水平明显低于阳性对照组(P<0.01),而且MSCs比例越高,其抑制作用越强(P<0.01)。经MSCs作用后,T细胞分泌IFN-γ的水平明显降低,而分泌IL-4的水平明显升高,且这种作用随MSCs比例的增加而增强(P<0.01)。MSCs能够抑制在体外培养过程中T细胞的自发凋亡。与MSCs共培养后,T细胞对L1210细胞的杀伤活性和单独培养组相比明显下降(P<0.05)。结论:MSCs能够抑制T细胞增殖反应和CTL活性,该作用可能与MSCs改变T细胞分泌细胞因子水平有关,但与诱导T细胞凋亡无关。Objective：To study the immunoregulatory effects of rat bone marrow mesenchymal stem cells on T cells in vitro.Methods：MSCs from bone marrow of rat were isolated and cultured in vitro.The morphological characterization of MSCs was observed after Wright-Giemsa staining and the typical molecules on the cell surface were identified by flow cytometry（FCM）.ConA was used as stimuli origin.Effect of MSCs on proliferation of T cells was determined by MTT method.Effects of MSCs on IFN-γand IL-4 secretion of T cells were determined by ELISA assay.Effect of MSCs on apoptotic level of T cells was studied by FCM.The effect of MSCs on cytotoxicity of CTL was investigated by MTT method.Results：Proliferation of T cells were decreased obviously after cocultivated with different numbers of MSCs in a dose-dependent manner compared with positive control（P〈0.01）.The level of IFN-γ secreted by T cells was decreased while the level of IL4 was increased significantly in a dose-dependent manner（P〈0.01）.The apoptosis of T cells was not induced by MSCs.After treated by MSCs,cytotoxicity of CTL was impaired compared with the control group（P〈0.05）.Conclusion：MSCs could inhibit the proliferation of T cells and the cytotoxicity of CTL in vitro.These effects were related to inducing cytokine secretion but not apoptosis of T cells.

关 键 词：大鼠 间充质干细胞 免疫调节 T细胞 细胞毒性T细胞 

分 类 号：R392.12[医药卫生—免疫学]