MICA新等位基因MICA*002:04的DNA序列鉴定及分析  

Identification of a novel MICA allele MICA*002:04 by sequencing based typing and clone sequencing

作　　者：谭茵[1] 黄颖烽[2] 徐秀章[3] 叶欣[3] 魏亚明[2] 古维立[2] 

机构地区：[1]广州医学院基础学院,广州510182 [2]广州医学院附属广州市第一人民医院,广州510180 [3]广州血液中心,广州510095

出　　处：《中国免疫学杂志》2011年第5期434-437,445,共5页Chinese Journal of Immunology

基　　金：广东省自然科学基金(9151006001000009);广州市医药卫生科技一般引导项目(2009-YB-137)

摘　　要：目的:直接测序法及外显子3 DNA片段克隆测序定确认MICA新的等位基因MICA*002:04。方法:用组特异性引物分别扩增MICA基因,采用Sequence Base Typing(SBT)法分型技术及PCR片段克隆测序法对MICA多态性基因的外显子2、3、4、5进行双向测序。结果:发现1个与MICA*002:01序列相近的新等位基因,在外显子3上有1个碱基位置与国际通用MICA数据库不相符。该基因与MICA*002:01相比在外显子3的碱基位置486出现突变(C→A),密码子位置20由GCC→GCA,相应编码氨基酸是同义突变。结论:DNA测序结果表明该基因序列为新的MICA等位基因,已提交GenBank,于2010年9月被世界卫生组织HLA因子命名委员会正式命名为MICA*002:04。Objective：To identify of a novel MICA allele MICA＊002：04 by sequencing Based Typing and Clone Sequenting.Methods：Samples were tested by Sequence Based Typing（SBT） as routine MICA allele typing in a research project supported by Guangdong provincial fund.MICA-PCR DNA fragment was inserted into a pMD-T vector and re-sequenced to confirm the typing results.Results：The DNA sample of a lung cancer patient was found mismatched in MICA gene bank database.Sequence Based Typing（SBT） and clone sequence results showed that there was a mutation point at position 486 on exon 3,changing from C→A,resulting in codon 20 changing from GCC to GCA.The amino acid sequence has not been changed since it is a silent mutation.The sequence of MICA＊002：04 allele was submitted to GenBank and its submission number is HWS10011132.Conclusion：The name MICA＊002：04 has been officially assigned by the WHO Nomenclature Committee in September 2010.This follows the agreed policy that,subject to the conditions stated in stated in the most recent Nomenclature Report（Marsh et al.2010） names will be assigned to new sequences as they are identified.Lists of such new names will be published in the following WHO Nomenclature Report.

关 键 词：DNA测序分型 MICA 新等位基因MICA*002 

分 类 号：Q755[生物学—分子生物学] R392.2[医药卫生—免疫学]