痂囊腔菌素A与肌红蛋白相互作用的光谱研究  

Spectroscopic Studies on the Interaction of Elsinochrome A with Myoglobin

作　　者：马菲[1] 周林[1] 王薇[1] 冯玉英[1] 周家宏[1] 王兴和[2] 沈健[1] 

机构地区：[1]江苏省生物功能材料重点实验室,分析测试中心,南京师范大学,江苏南京210046 [2]南京师范大学物理与科学技术学院,江苏南京210046

出　　处：《光谱学与光谱分析》2011年第6期1601-1605,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(20603018);江苏省教育厅自然科学基金项目(04KJD150110);江苏省光电技术重点实验室开放基金项目(KJC09)资助

摘　　要：研究了痂囊腔菌素A(EA)与肌红蛋白(Mb)的相互作用。通过紫外-可见吸收光谱研究发现痂囊腔菌素A与肌红蛋白在避光与光照两种条件作用模式存在明显差异;痂囊腔菌素A对肌红蛋白的内源荧光有猝灭作用,形成了新的配合物,属于静态猝灭;热力学计算结果表明痂囊腔菌素A与肌红蛋白之间以疏水作用力结合。同步荧光光谱研究了痂囊腔菌素A对肌红蛋白二级结构的影响。The interaction between Elsinochrome A（EA） and myoglobin（Mb） was investigated using UV-Vis and fluorescence spectroscopy.The results suggested that there was a strong interaction between EA and Mb.In the dark,the interaction occurred on the surfaces amidic acid of Mb,but when illuminated,the interactions happened both on amidic acid and the interior structure of hemachrome of Mb.According to the values of the quenching constant,the thermodynamics parameters,the binding constants and the binding sites,it was showed that the binding interaction of EA and Mb was mainly hydrophobic in nature and the quenching mechanism was static quenching procedure.The change in the micro-circumstance of aminos of myoglobin was studied by synchronous fluorescence spectroscopy.

关 键 词：痂囊腔菌素A 肌红蛋白 紫外-可见吸收光谱 荧光光谱 

分 类 号：O657.3[理学—分析化学]