JAZF1基因过表达对鼠肝细胞糖脂代谢相关基因表达的影响  

Effects of over-expression of JAZFl gene on lipid and glucose metabolism-related gene expression in hepatic cells

作　　者：卢春敏[1] 李伶[1] 杨刚毅[2] 杨艳[1] 李钶[2] 孙滨[1] 苗宗玉[1] 

机构地区：[1]重庆医科大学医学检验系临床生化教研室和临床检验诊断学教育部重点实验室,400016 [2]重庆医科大学附属第二医院内分泌科

出　　处：《中国糖尿病杂志》2011年第5期366-370,共5页Chinese Journal of Diabetes

基　　金：国家自然科学基金资助项目(30871199;30771037;30971388;81070640);教育部博士点基金(20105503110002)

摘　　要：目的探讨JAZF1过表达对鼠肝细胞IAR-20及Hepa1-6糖脂代谢相关基因的影响。方法克隆鼠JAZF1编码区并构建pIRES2-EGFP-JAZF1表达载体,利用脂质体法将目的基因转染到鼠肝细胞IAR20及Hepa1-6中,采用实时荧光定量PCR测定各处理组JAZF1 mRNA表达情况,以及糖脂代谢相关基因SREBP1、ACC、FAS、HSL、PPARα、ATGL、GluT-1、GluT-4和细胞因子FGF 21mRNA的变化情况。结果 JAZF1基因在鼠肝细胞中过表达导致脂代谢基因SREBP1,ACC,FAS表达降低(P<0.01),PPARα和HSL表达增加(P<0.01),对ATGL无影响(P>0.05),使糖转运相关基因GluT-1表达增加(P<0.01),GluT-4无显著变化(P>0.05),对FGF-21无影响(P>0.05)。结论 JAZF1过表达可以抑制肝细胞的脂肪生成,促进脂肪分解,并可能增加基础葡萄糖转运。Objective To investigate the role of JAZF1（juxtaposed with another zinc finger gene 1） in the regulation of glucose and lipid metabolism in hepatic cells. Methods The JAZF1 gene was transfected into hepatic IAR-20 cells and hepatoma carcinoma Hepa1-6 cells using vector-mediated pIRES2- EGFP-JAZF1. Expression of several genes which implicated in metabolism was measured using fluorescent quantitative PCR. Results In JAZF1-overexpressing hepatic cells, cellular SREBP1, ACC and FAS levels were down-regulated as compared with the control cells （P〈0. 01）. The levels of PPARα and HSL mRNA were significantly increased （all P〈 0. 01 ）. But there was no significant difference in the expression of ATGL gene between JAZFl-overexpressing cells and control cells （P〉0. 05）. In addition, the transcriptions of GluT-1 was significantly higher as compared with the control （P〈0. 001）, but there was no statistical difference in the expression of GluT-4（P〉0. 05）. Compared with the controls, JAZF1 had no effects on FGF-21 mRNA in cells. Conclusion Overexpression of JAZF1 promotes lipolysis, inhibits adipogenesis and increases basal glucose transport in hepatic cells.

关 键 词：JAZF1 IAR-20细胞 Hepal-6细胞 脂类代谢 葡萄糖代谢 

分 类 号：R587.1[医药卫生—内分泌]