鸡传染性支气管炎病毒河南流行毒株S1基因的变异分析  

Variation analysis of S1 gene of avian infectious bronchitis virus epidemicstrains isolated from chicken flocks in Henan Province

作　　者：徐雪[1] 赵军[1] 王川庆[1] 王泽霖[1] 杨霞[1] 周欣[1] 迪丽拜尔.阿木提 

机构地区：[1]河南农业大学禽病研究所,河南郑州450002 [2]阿克苏职业技术学院,新疆阿克苏843000

出　　处：《浙江大学学报（农业与生命科学版）》2011年第3期263-274,共12页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：中德合作资助项目(43006167);国家农业科技成果转化基金资助项目(2008GB2D000182)

摘　　要：经鸡胚接种、反转录-聚合酶链式反应(RT-PCR)、气管环培养等方法从河南不同地区发病鸡群中先后共分离、鉴定出7株鸡传染性支气管炎病毒(IBV).应用RT-PCR方法对7株病毒以及IBVH120疫苗株的S1基因全序列进行扩增,并对其进行克隆测序及序列分析.结果显示:8株IBV的S1基因全长分为4类,分别是1 632、1 6201、617和1 611 bp;序列相互之间存在多位点变异,同时存在型特异性的插入和缺失;其S蛋白裂解位点序列模式有HRRRR、RRFRR和RRSRR 3种;同源性及遗传进化分析显示Jin-13为呼吸型毒株,YI为ArkDPI型肾型毒株,HN/SG株与国内其他肾型分离株亲缘关系均较远;河南肾型分离株HN/HL、XP/1/09、XP/3、WZL与山东肾型分离株之间的同源性较高且其亲缘关系最近,而与河南HN99株的亲缘关系较远.说明河南地区存在不同的IBV流行毒株,这些流行毒株在主要保护性抗原蛋白S1基因上发生了一定的变异.Seven isolates of infectious bronchitis virus（IBV） were obtained and identified from clinical infected chicken flocks in Henan Province by using the techniques of chicken embryo inoculation,reverse transcription polymerase chain reaction（RT-PCR） and tracheal organ culture（TOC）.Spike protein gene of 7 isolates and vaccine strain H120 was amplified by RT-PCR and sequenced.The result showed that the sizes of the whole length for S1gene of 8 IBV strains were divided into four groups：1 632,1 620,1 617 and 1 611 bp,respectively.There were multisite mutations among these sequences in addition to type-specific insertion and deletion.The analysis of deduced amino acid sequences showed that sequential patterns of S protein cleavage sites of 8 IBV strains fell into three categories： HRRRR,RRFRR and RRSRR.Homology and phylogenetic analysis showed that Jin-13 isolate was respiratory-type of IBV,and YI isolate was ArkDPI-type,and HN/SG isolate had a distant relationship to other domestic nephropathogenic-type isolates else,and HN/HL,XP/1/09,XP/3 and WZL isolates had a close relationship to Shandong nephropathogenic-type isolates but had a distant relationship to HN99 isolate.All results above indicate that there are different IBV epidemic isolates in Henan Province,and certain mutations at the molecular level have occurred in S1gene.

关 键 词：传染性支气管炎病毒 肾型 呼吸型 序列分析 S蛋白 裂解位点 遗传进化分析 

分 类 号：Q78[生物学—分子生物学] S852.659.6[农业科学—基础兽医学]