妊娠奶牛PAPPA和PAG mRNA表达规律的研究  

Preliminary Study on PAPPA and PAG mRNA Expression in Cows

作　　者：郭宁[1] 李莲[1] 周璇[1] 崔群维[1] 王根林[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095

出　　处：《中国牛业科学》2011年第3期1-5,共5页China Cattle Science

基　　金：国家科技部奶业支撑计划(2006BAD04A12);江苏省科技支撑计划(BE2008306-2)共同资助

摘　　要：[目的]分析PAPPA(妊娠相关血浆蛋白A)和PAG(妊娠相关糖蛋白)mRNA在不同妊娠时期奶牛外周血的表达规律。[方法]提取奶牛空怀期和妊娠30 d、60 d、90 d、150 d和240 d血浆中游离RNA,RT-PCR扩增PAPPA和PAG的基因,分析其在妊娠期的相对表达水平,并研究其表达规律。[结果]PAG基因在空怀组和妊娠牛均有表达,在牛妊娠240 d时相对表达水平达到高峰;PAPPA从妊娠30 d时表达水平开始升高,并在妊娠150 d时达到最高水平。[结论]本研究为深入探讨奶牛PAPPA和PAG基因调控规律和早期妊娠诊断提供了参考。【Objective】The expression regularity was analyzed of PAPPA（Pregnancy associated plasma protein A） and PAG（Pregnancy associated glycoprotein） mRNA in peripheral blood of Holstein cows.【Method】Extract free RNA from the plasma of un-pregnant and pregnant cows at the 30 d,60 d,90 d,150 d and 240 d of pregnancy.PAPPA and PAG genes were amplified by RT-PCR.【Result】PAG was detected in both pregnant cows and non-pregnant cows.The relative expression level rose to the highest at the 150 d of pregnancy.PAPPA expression began to increase at 30 d and reached to the highest at 150 d.This result provided the reference for further exploring the regulation of PAPPA and PAG and for early pregnancy diagnosis in cows.

关 键 词：奶牛 PAPPA PAG 基因表达 

分 类 号：S814.1[农业科学—畜牧学]