犬GM-CSF基因的克隆、序列分析及蛋白结构预测  

Cloning and sequence analysis of Canis GM-CSF gene and prediction of protein structure

作　　者：刘杰[1] 蒋成砚[1] 胡俊杰[1,2] 全舒舟[1] 雷泉[1] 

机构地区：[1]红河学院生命科学与技术学院,云南蒙自661100 [2]云南大学生命科学学院,云南昆明650091

出　　处：《黑龙江畜牧兽医》2011年第4期16-19,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(30770257);红河学院生物化学与分子生物学重点学科建设项目(071010)

摘　　要：为了研究犬粒细胞/巨噬细胞集落刺激因子(GM-CSF)的作用,试验根据GenBank上发表的犬(Canis familiaris)GM-CSF cDNA基因序列设计1对引物,采用RT-PCR技术,以ConA刺激的犬外周血淋巴细胞为材料,从总RNA中扩增出犬GM-CSF基因。结果表明:扩增片段长为435 bp,编码144个氨基酸;生物软件分析结果表明,该序列与猕猴的亲源性更近,与已知人、马、牛、野猪、豹猫、鸡等序列的同源性较低。In order to research the function of canine GM-CSF,based on the published nucleotide sequence of canine（Canis familiaris）GM-CSF gene,a pair of primers were designed and synthesized,the total RNA was isolated from ConA-stimulated canine peripheral blood T cells,then the CaGM-CSF gene was cloned by RT-PCR technique.The result showed that cloned GM-CSF cDNA was 435 bp in size and encoded a polypeptide of 144 amino acids.Compared CaGM-CSF with published on GenBank.The high identity between CaGM-CSF and Rhesus macaque was found,but low identity was found among canine GM-CSF,human GM-CSF,horse GM-CSF,buttle GM-CSF,pig GM-CSF,cat GM-CSF,chicken GM-CSF and so on.The cloned CaGM-CSF gene may construct a basis on further research on protein fuction.

关 键 词：犬 GM-CSF基因 克隆 序列分析 

分 类 号：S814.8[农业科学—畜牧学]