大豆芽期耐盐和耐低温位点的遗传重叠  

Genetic Overlap Between Salt and Low-Temperature Tolerance Loci at Germination Stage of Soybean

作　　者：邱鹏程[1,2] 张闻博[1,2] 蒋洪蔚[1] 刘春燕[1] 李灿东[3] 范冬梅[1] 曾庆力[1] 韩冬伟[4] 胡国华[1,5] 陈庆山[2] 

机构地区：[1]黑龙江省农垦科研育种中心,哈尔滨150090 [2]东北农业大学农学院,哈尔滨150030 [3]黑龙江省农业科学院佳木斯分院,黑龙江佳木斯154007 [4]黑龙江省农业科学院齐齐哈尔分院,黑龙江齐齐哈尔161006 [5]国家大豆工程技术研究中心,哈尔滨150050

出　　处：《中国农业科学》2011年第10期1980-1988,共9页Scientia Agricultura Sinica

基　　金：国家转基因专项(2009ZX08009-013B);国家"948"项目(2006-G1A)

摘　　要：【目的】利用大豆回交导入系为材料定位芽期耐盐性QTL和耐低温QTL位点,并对芽期耐盐和耐低温的QTL位点进行遗传重叠分析。这些重叠QTL的辅助选择可用于培育芽期耐盐且耐低温的大豆品种,提高大豆抗逆育种效率。【方法】将Harosoy导入到以红丰11为背景的回交导入系中,对BC2F4世代进行大豆芽期耐盐性和耐低温筛选,获得的超亲导入系用卡方检测和方差分析的方法定位QTL。【结果】芽期耐盐性筛选获得48个耐盐选择导入系,采用单项方差分析和卡方检测共定位了22个控制芽期耐盐QTL。芽期耐低温筛选获得40个耐低温选择导入系,采用2种遗传分析方法共定位到15个控制芽期耐低温QTL。分布于A1、B2、C2、E、J和O连锁群上的7个位点(Sat_271、Satt556、Satt726、Satt640、Satt411、Satt529和Sat_108)是大豆芽期盐胁迫和低温条件下共同检测到的。【结论】从整体上,31.81%大豆芽期耐盐性和耐低温位点存在遗传重叠。【Objective】The main purpose of this paper was to map the QTLs related with salt tolerance and low-temperature tolerance at germination stage with backcross introgression lines（ILs）,and analyze the genetic overlap between them.These overlapping QTL loci could be used to breed soybean varieties with both salt tolerance and low-temperature tolerance,improve efficiency of stress-tolerance breeding.【Method】A primary backcross introgression lines（ILs） were constructed with Hong-feng11 as recurrent parent and Harosoy as donor parent.Then 48 individuals from BC2F4 introgression populations were obtained after screening in 1.75% NaCl solution,and 40 individuals were screened out after 6℃ low-temperature treatment comparing with the Hong-feng11 at germination stage.The QTL identification of the germination stage was conducted by chi-square test and analysis of variances with the two introgression populations.【Result】There were 22 QTL located with BC2F4 introgression populations after salt stress,and 15 QTL were mapped after low-temperature stress.Seven overlapping QTLs between salt tolerance and low-temperature tolerance were detected on 6 linkage groups at germination stage.【Conclusion】In total,there were 31.81% of salt tolerance and low-temperature tolerance loci existed genetic overlap.

关 键 词：大豆 回交导入系 耐盐性 耐低温性 遗传重叠 

分 类 号：S565.1[农业科学—作物学]