RP-HPLC法同时测定中药关白附中关附甲素、关附壬素和关附辛素含量  

Simultaneous Determination of Guanfu Base A,Guanfu Base I and Guanfu Base H in Radix Aconitum coreanum(Lévl) Rapaics by RP-HPLC

作　　者：吴琼[1] 杨春华[2,3] 刘静涵[4] 秦民坚[1] 

机构地区：[1]中国药科大学中药资源教研室,江苏南京210009 [2]中国药科大学分析测试中心,江苏南京210009 [3]教育部药物质量与安全预警重点实验室,江苏南京210009 [4]中国药科大学天然药物化学教研室,江苏南京210009

出　　处：《药学进展》2011年第5期230-233,共4页Progress in Pharmaceutical Sciences

基　　金：国家科技重大专项(2009ZX09103-345)

摘　　要：目的:建立同时测定关白附中3种二萜生物碱———关附甲素、关附壬素和关附辛素的RP-HPLC分析方法。方法:色谱柱:Zorbax Eclipse XDB-C8柱(2.1 mm×150 mm,5μm),流动相:庚烷磺酸钠缓冲液(加入0.2%三乙胺,磷酸调至pH 3)-乙腈(梯度洗脱),柱温:25℃,流速:0.2 mL.min-1,检测波长:205 nm。结果:3种生物碱的相邻色谱峰分离度大于1.5,关附壬素、关附甲素和关附辛素质量浓度分别在0.100～0.502、0.100～0.498和0.100～0.500 g.L-1的范围内与峰面积呈良好的线性关系(r=0.999 9),其平均加样回收率(n=9)分别为97.29%、101.46%、102.33%。结论:本法分离效果和重复性好,简便易行,可用于关白附药材中3种生物碱的同时定量分析及药材质量控制。Objective：To establish a RP-HPLC method for the simultaneous determination of three major diterpenoid alkaloids,including guanfu base A（GFA）,guanfu base I（GFI）and guanfu base H（GFH） in Radix Aconitinum coreanum（Lévl） Rapaics.Methods：A Zorbax Eclipse XDB-C8 column（2.1 mm×150 mm,5 μm） and sodium heptanesulfonate（adding 0.2 % triethylamine,pH 3.00 adjusted with phosphoric acid）-acetonitrile as mobile phase were used at a flow rate of 0.2 mL ·min-1 and with a column temperature of 25 ℃ and a detection wavelength of 205 nm by gradient elution.Results：A good resolution（R1.5） was achieved for three diterpenoid alkaloids.Good linearities（r=0.999 9） of peak area-concentration in the ranges of 0.100-0.502,0.100-0.498 and 0.100-0.500 mg ·L-1 respectively for GFI,GFA and GFH were shown.The average recoveries（n=9） of GFI,GFA and GFH were 97.29 %,101.46 % and 102.33 % respectively.Conclusion：The method is simple and good for separation and repeatability.It can be used for the simultaneous quantitative determination of three diterpenoid alkaloids in Radix Aconitium coreanum（Lévl） Rapaics and the quality control of the herbs.

关 键 词：关白附 关附壬素 关附甲素 关附辛素 反相高效液相色谱 

分 类 号：O657.72[理学—分析化学] R284.2[理学—化学]