水杨酸钠对豚鼠下丘镁离子的影响  

Effects of salicylate on magnesium in the inferior colliculus of guinea pigs

作　　者：李涛[1] 刘俊秀[1] 张自品[2] 毛兰群[2] 马芙蓉[1] 

机构地区：[1]北京大学第三医院耳鼻咽喉头颈外科,北京100191 [2]中国科学院化学研究所活体分析化学院重点实验室,北京100190

出　　处：《中国耳鼻咽喉头颈外科》2011年第4期181-184,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家自然科学基金(81070788;30500556;90813032);北京市自然科学基金(7082100)联合资助

摘　　要：目的研究腹腔注射水杨酸钠对豚鼠下丘Mg2+浓度的影响,探讨Mg2+在耳鸣发生机制中的作用。方法 16只豚鼠随机分为实验组(n=8),腹腔注射水杨酸钠下丘组;对照组1(n=4),腹腔注射生理盐水下丘组;对照组2(n=4),腹腔注射水杨酸钠尾壳核组。利用活体微透析取样在线电化学检测技术,活体实时动态检测Mg2+浓度的变化。结果实验组豚鼠腹腔注射水杨酸钠约1.5 h后,下丘Mg2+浓度显著性下降,降至原浓度的(62.2±16.3)%;对照组1、2给药前、后目标脑区Mg2+浓度没有显著性变化。结论水杨酸钠注射后下丘Mg2+浓度的显著性降低可能与水杨酸钠导致的耳鸣有关。OBJECTIVE To study the change of magnesium（Mg2＋）in the inferior colliculus（IC）of guinea pigs induced by intraperitoneal sodium salicylate injection during the process of tinnitus.METHODS 16 guinea pigs were randomly divided into 3 groups： the experimental group（n=8）,studying the changes in IC after intraperitoneal sodium salicylate injection;control group 1（n=4）,studying the changes in IC after intraperitoneal physiological saline injection;control group 2（n=4）,studying the changes in the nucleus caudatus putamen after intraperitoneal sodium salicylate injection.The concentration of Mg2＋ was continuously monitored by in vivo microdialysis coupled with online selective electrochemical detection.RESULTS In the experimental group,the concentration of Mg2＋ in IC decreased significantly to（62.2±16.3）% of the basal level 1.5 hours after intraperitoneal injection of sodium salicylate;In control group 1 and 2,there was no significant changes of the Mg2＋ in the target brain regions after intraperitoneal injection.CONCLUSION The significant decrease of Mg2＋ in IC may be involved in the process of tinnitus induced by salicylate.

关 键 词：水杨酸钠 耳鸣 镁 活体微透析 在线电化学检测 

分 类 号：R965[医药卫生—药理学]