云南保山、普洱带绦虫mtDNA-ND1基因序列测定及分析  

Gene sequencing and analysis of mtDNA-ND1 in Taenia cestodes from Baoshan and Puer of Yunnan province

作　　者：刘爱波[1] 杨毅梅[1] 

机构地区：[1]云南大理学院下关校区基础医学院寄生虫学教研室,大理671000

出　　处：《中国人兽共患病学报》2011年第4期340-342,共3页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(30860252)

摘　　要：目的对云南保山、普洱地区带绦虫标本进行鉴定。方法选取带绦虫成虫节片,抽提虫体基因组DNA,PCR扩增mtDNA-ND1基因序列,并测序;结合GenBank中已知的亚洲带绦虫、牛带绦虫、猪带绦虫mtDNA-ND1基因序列,经DNA MAN软件处理后构建系统发育树状图与同源树状图。结果带绦虫系统发育树与同源树状图显示P1、P2、B3、B6、B7、ND与N2的同源性最近。B1、B2、B4、B5、YZ与Y1的同源性最近。ZD与Z3的同源性最近。结论云南保山存在亚洲带绦虫与牛带绦虫;普洱存在牛带绦虫。mtDNA-ND1基因序列可用于三种带绦虫的分类鉴定。To identify the Taenia cestode specimens collected from Baoshan and Puer regions of Yunnan province,the adult Taenia cestode segments were selected,genomic DNA was extracted,and mtDNA-ND1 gene sequence was amplified by PCR and sequenced.Combined with the known mtDNA-ND1 gene sequences of Taenia asiatica,Taenia saginata and Taenia solium in GenBank,phylogenetic tree and homologous tree were constructed by using DNA MAN software.Taenia cestode phylogenetic tree and homologous tree showed homology of P1,P2,B3,B6,B7 and ND were mostly close to N2.Homology of B1,B2,B4,B5 and YZ were mostly close to Y1.Homology of ZD was mostly close to Z3.Taenia asiatica and Taenia saginata exist in Baoshan of Yunnan,and Taenia saginata exists in Puer.MtDNA-ND1 gene sequence could be used for identification of three types of Taenia cestode.

关 键 词：带绦虫 鉴定 mtDNA-ND1 基因序列 

分 类 号：R383.3[医药卫生—医学寄生虫学]