甲醇产纤维素菌株的静态发酵动力学研究  

Study on Static Fermentation Kinetics of Bacterial Cellulose-Producing Strain Using Methanol

作　　者：夏露[1] 朱艳彬[1] 刘振鸿[1] 李小维[1] 

机构地区：[1]东华大学环境科学与工程学院,上海201620

出　　处：《生物技术》2011年第2期70-73,共4页Biotechnology

基　　金：国家自然科学基金青年项目("生物复合絮凝剂吸附絮凝过程;形态及机理研究";No.50908039);生态纺织教育部重点实验室开放课题("利用高浓度甲醇废水制备细菌纤维素的研究";No.ECO-KF-2007-09);高等学校博士学科点专项科研基金项目("高效生物絮凝活性物质作用机制研究";No.20070255019);上海市重点学科项目(No.B604)资助

摘　　要：目的:为实现甲醇资源化产细菌纤维素发酵过程的优化,研究纤维素生产菌株—木醋杆菌(Gluconacetobacter xylinus)的静态发酵动力学特性。方法:将木醋杆菌接入甲醇浓度分别为2.7%和4.5%的培养基中驯化,根据Logistic方程和Luedeking-Piret方程,研究周期为13d的静态发酵动力学过程。结果:确定静态发酵过程的菌体生长、细菌纤维素合成、底物消耗的动力学参数,得到动力学方程,拟合试验值与模型值,得到甲醇模拟废水培养基平均拟合误差为16%,略高于基础培养基的14%。结论:利用甲醇产纤维素的模型方程可预测菌浓、产物浓度及底物消耗规律,实现静态发酵过程的优化。Objective： For optimizing methanol into resource,the static fermentation kinetics of bacterial cellulose-producing strain Gluconacetobacter xylinus was analyzed.Method：After naturalizing in the mediums which methanol concentration were 2.7% and 4.5%,and based on the Logistic quotation and Luedeking-Piret equation,the progress of static fermentation for 13 days was investigated.Result：The kinetic parameters and models were proposed on microorganism growth,product formation and substrate consumption.With the evaluated model parameters,the fitting error using methanol wastewater simulated culture medium was 16% which was slightly above than that using base culture medium of 14%.Conclusion： The regular of fermentation including concentration of bacterium and product and substrate could be forecasted,and it was positive to realize the optimization of static fermentation progress.

关 键 词：细菌纤维素 甲醇 动力学模型 发酵 

分 类 号：X703[环境科学与工程—环境工程]