乳腺导管内乳头状瘤高危型人乳头瘤病毒感染状况的临床研究  

Clinical research of high risk human papillomavirus infection in intraductal papilloma of breast

作　　者：黄建棋[1,2] 吕艳蓉[1] 王磊一[3] 刘振平[3] 马婷婷[1] 江立玉[1] 张凯[1] 高海东[1] 杨其峰[1] 马榕[1] 

机构地区：[1]山东大学齐鲁医院乳腺外科,山东济南250012 [2]浙江省嘉兴市第一医院乳腺病科,浙江嘉兴314000 [3]山东大学齐鲁医院基因室,山东济南250012

出　　处：《中国现代普通外科进展》2011年第3期190-192,203,共4页Chinese Journal of Current Advances in General Surgery

基　　金：山东省自然基金资助项目(ZR2009CM007)

摘　　要：目的:研究乳腺导管内乳头状瘤中是否存在高危型人乳头瘤病毒(HPV)的感染。方法:采用二代杂交捕获技术(HC2)定量检测30例乳腺导管内乳头状瘤患者的肿瘤组织或乳头溢液和18例乳腺纤维腺瘤患者肿瘤组织中13种高危型HPV DNA的含量。结果:30例乳腺导管内乳头状瘤组织或溢液中有3例呈阳性(检测值>1),阳性率为10%,而18例乳腺纤维腺瘤患者肿瘤组织中未发现高危型HPV的感染。30例乳腺导管内乳头状瘤患者中中央型导管内乳头状瘤11例,周围型导管内乳头状瘤19例,3例阳性者均为中央型,中央型导管内乳头状瘤的HPV阳性率与周围型导管内乳头状瘤存在明显差异(P<0.05)。结论:乳腺导管内乳头状瘤存在高危型HPV的感染,HPV感染的来源可能是通过乳管的开口进入乳腺,不能支持高危型HPV感染与乳腺导管内乳头状瘤发展及癌变有关的观点。Objective： To examine the infection of 13 high risk human papilloma-virus（HR-HPV） in Intraductal papilloma of breast.Methods： Hybrid Capture Ⅱ technique was done on 30 nipple discharges samples or tumor tissues from the patients with intraductal papilloma of breast and 18 tissues from the patients with breast fibroadenoma,to examine the infection of high risk human papilloma-virus DNA（HR-HPV DNA）.Results： In 30 patients with intraductal papilloma of breast,there are 3 positive of HR-HPV DNA,positive rate is 10%.No HR-HPV DNA was found in patients with breast fibroadenoma.The 3 positives are all the Central-type intraductal papilloma.There are clear difference between the positive rate of HR-HPV of the Central-type intraductal papilloma and Peripheral intraductal papilloma（P0.05）.Conclusion： HR-HPV DNA infection exists in intraductal papilloma,and HR-HPV may infect the breast through the duct opening.Our results can＇t support that HR-HPV is associated with the development and cancerization of intraductal papilloma.

关 键 词：乳腺导管内乳头状瘤 高危型人乳头状瘤病毒 二代杂交捕获试验 

分 类 号：R655.8[医药卫生—外科学]