量子点免疫荧光双标法检测银屑病皮损中Th17细胞  

Using Quantum Dots Double-lable Immunofluorescence to Study Skin Lesions Localization of Th17 Cells in Psoriatic Patients

作　　者：郑道城[1] 常树霞 黄莉宁[1] 陈文静[1] 邱苗芳 薛耀华[1] 孙希凤[1] 黎明[1] 陈永锋[1] 

机构地区：[1]广东省皮肤性病防治中心,广东广州510500 [2]深圳市龙岗区慢性病防治院,广东深圳518172 [3]广州市妇女儿童医疗中心,广东广州510180

出　　处：《皮肤性病诊疗学杂志》2011年第2期97-100,共4页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：广东省医学科学技术研究基金(A2009126)

摘　　要：目的:探讨量子点免疫荧光双标法检测银屑病皮损中Th17细胞的可行性。方法:用量子点免疫荧光双标法检测银屑病患者皮损区及正常人皮肤组织中Th17细胞的表达。结果:银屑病患者皮损组织中有一定量Th17细胞的浸润(4.66%±2.65%),主要集中在真皮浅层,血管周围;而正常皮肤组织中未见Th17细胞,仅有微量CD4+T细胞表达。结论:量子点免疫荧光双标法成功的检测到银屑病皮损中Th17细胞高表达,为今后量子点在皮肤病中的应用和发展提供了依据。Objective：To investigate the pathogenic effects of Th17 cells in psoriasis. Methods：Using quantum dots doublelable immunofluorescence to analyze the localization and quantitation of Th17 cells in skin lesions between psoriasis and normal contrals.Results：Th17 cells was detected in skin lesions of psoriasis（4.66±2.65）%,those were major in dermis layer around dlood vessel,but not in those of normal contrals.Conclusion：Made use of quantum dots doublelable immunofluorescence to invest that the higer expression of Th17 cells in the lesion from psoriatic patients and provided the basis for quantum dots at skin disease＇s application and the development.

关 键 词：银屑病 TH17细胞 量子点 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]