Kv1.3在动脉粥样硬化大鼠主动脉的表达变化  

Expression profile of voltage-gated potassium channel Kv1.3 in aorta of atherosclerosis rats

作　　者：王焱[1] 李桂阳[1] 李枚娟[1] 巩燕[1] 肖国胜[1] 常贺[1] 陈航姿[2] 姚路明[2] 王维嘉[2] 

机构地区：[1]厦门大学附属中山医院心内科,厦门361004 [2]厦门大学生命科学院

出　　处：《上海医学》2011年第3期186-189,I0001,共5页Shanghai Medical Journal

基　　金：国家自然科学基金资助项目(30871045)

摘　　要：目的探讨动脉粥样硬化(AS)大鼠主动脉病变局部离子通道Kv1.3的表达水平及作用。方法雄性Wistar大鼠16只,随机分为两组:正常组(8只,予普通饮食+0.9%氯化钠溶液)和AS组(8只,予高脂饮食+维生素D3负荷)。采用组织病理学检查,观察主动脉粥样硬化病变。采用实时定量反转录-聚合酶链反应(RT-PCR)和Western印迹法检测主动脉病变局部Kv1.3和白细胞介素(IL)-2、干扰素(IFN)-γ的表达水平。结果组织病理学检查证实纤维增生性AS斑块。AS组的Kv1.3mRNA为(31.48±8.64)×10-3,显著高于正常组的(3.28±0.79)×10-3(P=0.012)。AS组的Kv1.3、IL-2、IFN-γ蛋白表达量分别为0.691±0.067、0.611±0.055、0.759±0.050,均显著高于正常组的0.490±0.052、0.299±0.058、0.273±0.052(P值均<0.01)。结论 Kv1.3在主动脉粥样硬化病变局部的表达增高。Kv1.3可能在AS的发生和发展过程中发挥着重要的作用。Objective To investigate the expression of voltage-gated potassium channel Kv1.3 in the aorta of a rat model of atherosclerosis and its role in the progress of atherosclerotic plaque formation.Methods A total of 16 male Wistar rats were randomly divided into normal control(normal diet and saline)and atherosclerosis group(high lipid diet+Vitamin D3 overload),with 8 rats in each group.In 16 weeks later,all rats were killed after weighing,and their blood samples and aorta were collected.Pathological changes of the rat aortic artery were observed with HE staining.Real time RT-PCR and Western blotting analysis were used to determine the mRNA and protein expression of Kv1.3 and the protein expressions of interleukin(IL)-2 and interfereron(IFN)-γ.Results Pathological changes showed that fiber proliferative atherosclerotic plaques were found in the aorta of atherosclerosis group,with inflammatory cells infiltrating in the local lesion.Real time RT-PCR analysis showed that the expression of Kv1.3 mRNA in the aorta was increased significantly in the atherosclerosis rats than that in the controls([31.48±8.64]×10-3 vs.[3.28±0.79]×10-3,P<0.05).Western blotting analysis showed that the protein expression of Kv1.3,IL-2 and IFN-γ in the aorta were also increased significantly in the atherosclerotic rats than that in the controls(Kv1.3[0.691±0.067] vs.[0.490±0.052],IL-2 [0.611±0.055] vs.[0.299±0.058],IFN-γ [0.759±0.050] vs.[0.273±0.052],P<0.01 n=8).Conclusion The expression of Kv1.3 potassium channels is increased in the plaques of atherosclerotic rats.Kv1.3 may play an important role in the development and progression of atherosclerosis.

关 键 词：离子通道 动脉粥样硬化 细胞因子 免疫细胞 

分 类 号：R543.5[医药卫生—心血管疾病]