神香草总多糖的提取工艺研究  

Extraction Process of Polysaccharide from Hyssopus Offcinalis

作　　者：马雪萍[1,2] 马秀敏[1,3] 丁剑冰[1,3] 薛志琴[3] 阿依达尔[4] 谷茜[4] 田树革[1] 武新华[1,2] 

机构地区：[1]新疆医科大学附属中医院新疆国家中医临床研究基地,乌鲁木齐830000 [2]新疆医科大学药学院,乌鲁木齐830011 [3]新疆医科大学基础医学院,乌鲁木齐830011 [4]新疆医科大学,法医学乌鲁木齐830011

出　　处：《中国现代应用药学》2011年第5期437-439,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：新疆维吾尔自治区教育厅项目(XJEDU2008I17,XJEDU2009S50);新疆自治区自然科学基金项目(200821129);新疆医科大学大学生创新实验项目(CX2010004,CX2010006)

摘　　要：目的对维药神香草总多糖的最佳提取工艺进行研究。方法用正交试验法筛选最佳提取工艺,并用蒽酮-硫酸法测定多糖含量。结果最大吸收波长为620 nm,RSD=2.84%(n=3),神香草总多糖的最佳提取工艺为:料液比1∶25,提取次数2次,提取时间1 h,醇沉浓度为80%。结论该方法简单、快速、重复性好,为从神香草中提取多糖提供了参考依据。OBJECTIVE To study the optimum extraction process of polysaccharide from Hyssopus offcinalis.METHODS The optional ultrasonic extraction process was selected with the orthogonal design and the content of polysaccharides was determined by spectrophotometer.RESULTS λmax=620 nm,RSD=2.84%（n=3）,the optimum extraction process was as follows： the polysaccharide was extracted with ultrasonic technology for 1 hour with 25 times water for 2 times and alcohol concentration was 80%.CONCLUSION The experimental results provide the basis for the ultrasonic extraction process of polysaccharide from Hyssopus offcinalis.This optimum process is simple,stable and efficient.

关 键 词：正交试验 神香草 总多糖 

分 类 号：R284.2[医药卫生—中药学]