平喘汤对支气管哮喘大鼠肺组织IgE、TGFβ_1及TNF-α的影响  

Effect of Pingchuan Decoction on IgE,Transforming Growth Factor-beta 1 and Tumor Necrosis Factor-alpha in Lung Tissues of Rats with Bronchial Asthma

作　　者：冯高华[1,2] 朱虹[1] 孙峰[1] 贾永良[1] 

机构地区：[1]扬州大学医学院,江苏扬州225001 [2]张家港市中医院,江苏张家港215600

出　　处：《中药新药与临床药理》2011年第3期273-276,共4页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的探讨平喘汤对支气管哮喘大鼠的干预作用。方法将SD大鼠48只随机分为正常对照组、哮喘模型组、平喘汤低剂量组、平喘汤中剂量组、平喘汤高剂量组、阳性药物(博利康尼)对照组。采用卵蛋白致敏复制大鼠支气管哮喘模型,治疗组从实验开始第3周起,平喘汤高、中、低剂量组以2.6 mL/次、1.3 mL/次、0.7 mL/次剂量灌胃,阳性药物(博利康尼)对照组0.1 mL/次剂量灌胃,正常组及模型组均给予1.3 mL/次生理盐水灌胃,每天一次,连续4周。观察大鼠一般情况,大鼠肺组织IgE、TGFβ1及TNF-α的变化。结果哮喘组及各治疗组大鼠肺组织IgE、TGFβ1及TNF-α含量均高于正常组,差异显著(P<0.05);各治疗组大鼠肺组织IgE、TGFβ1及TNF-α含量均低于哮喘组,有显著性差异(P<0.05);与博利康尼组相比,平喘汤高剂量组大鼠肺组织IgE、TNF-α含量显著降低(P<0.01),而TGFβ1含量无统计学差异(P>0.05);平喘汤中剂量组大鼠肺组织IgE、TGFβ1含量高于博利康尼组(P<0.05),而TNF-α含量无统计学差异(P>0.05);平喘汤低剂量组大鼠肺组织IgE、TGFβ1、TNF-α含量均高于博利康尼组(P<0.01)。平喘汤高剂量组优于低剂量组。结论平喘汤能够通过下调大鼠肺组织IgE、TGFβ1及TNF-α含量,可降低气道高反应性、减轻气道炎症症状、控制或延缓气道纤维化进程。Objective To investigate the therapeutic effect of Pingchuan Decoction（PD） on rats with bronchial asthma.Methods Forty-eight SD rats were randomly divided into six groups： the normal group,the model group,low-dose PD（0.7 mL,once a day） group,medium-dose PD group（1.3 mL once a day）,high-dose PD group（2.6 mL once a day）,and positive medicine（Terbutaline Sulphate Tablets,0.1mL once a day） group.Except for the normal group,all rats in other groups were given intraperitoneal injection of ovalbumin（OVA） and forced inhalation of atomized OVA to induce bronchial asthma.From the 3rd week of modeling,the treatment groups were given the corresponding drugs according to the experimental design,and the normal group and model group were given 1.3 mL of normal saline（NS）,once a day for 4 weeks.The general state of the rats and the changes of IgE,TGFβ1 and TNF-α in lung tissues were observed.Results The levels of IgE,TGFβ1 and TNF-α in lung tissues of the model group and treatment groups were higher than those in the normal group,the difference being significant（P 0.05）.After treatment,IgE,TGFβ1 and TNF-α levels in lung tissues of treatment groups were lower than those in the model group,the difference being significant（P 0.05）.IgE and TNF-α levels in lung tissues of high-dose PD group were lower than those in the positive medicine group,there was significant difference between the two groups（P 0.01）.However,the difference of TGFβ1 between the two groups was insignificant（P 0.05）.IgE and TGFβ1 levels in lung tissues of medium-dose PD group were higher than those in positive medicine group（P 0.05）,but the difference of TNF-α level was insignificant between the two groups（P 0.05）.IgE,TGFβ1 and TNF-α in lung tissues of low-dose PD group were higher than those in positive medicine group,there was significant difference between the two groups（P 0.01）.The effect in high-dose PD group was superior to that in low-dose PD group.Conclusion PD has an effect on reducing a

关 键 词：支气管哮喘 气道高反应性 IGE 转化生长因子β1 肿瘤坏死因子α 

分 类 号：R285.5[医药卫生—中药学]