月腺大戟HPLC特征图谱研究  

Study on HPLC Fingerprint of Euphorbia Ebracteolata Hayata

作　　者：王灿坚[1,2] 江英桥[1] 林彤[1] 刘东辉[2] 严小红[1] 马双成[3] 

机构地区：[1]广州市药品检验所,广州510160 [2]广州中医药大学,广州510405 [3]中国药品生物制品检定所,北京100050

出　　处：《中药新药与临床药理》2011年第3期315-319,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家科技支撑计划药品安全关键技术研究(2006BAI14B00)资助课题-常见与重要药品安全标准研究(2006BAI14B01);广州市食品药品监督管理局科技计划项目(2010GZFDA015)

摘　　要：目的建立月腺大戟的HPLC特征图谱,为月腺大戟的质量控制提供依据。方法采用高效液相色谱法,以狼毒乙素为内参照物,采用Agilent ZORBAX SB-C1(8250 mm×4.6 mm,5μm)色谱柱,乙腈-水梯度洗脱,流速为1.0 mL.min-1,检测波长为226 nm,柱温20℃。结果获得了较为理想的月腺大戟药材HPLC特征图谱,确定了12个共有峰。结论该方法简便、准确,可靠,为评价月腺大戟药材的质量提供依据。Objective To establish HPLC fingerprint of Euphorbia ebracteolata Hayata.Methods 2,4-dihydroxy-6-methoxy-3-methylacetophenone was used as the internal standard substance.The reversed phase HPLC system consisted of a C18 column（250 mm × 4.6 mm,5 μm） and a mixture of acetonitrile-water as the mobile phase with gradient elution mode at the flow rate of 1.0 mL·min-1.The absorbance was monitored at 226 nm and the column temperature was 20 ℃.Results A satisfactory method for HPLC fingerprint determination of Euphorbia ebracteolata Hayata was established,12 characteristic peaks in the HPLC fingerprint were indicated.Conclusion The method is simple and accuracy with good reproducibility and can be used as a quality control method for Euphorbia ebracteolata Hayata.

关 键 词：月腺大戟 HPLC 特征图谱 

分 类 号：R284.1[医药卫生—中药学]