氟尿嘧啶合扶正抑瘤颗粒诱导肝癌细胞H_(22)凋亡的超微结构观察  

Ultrastructural Observation of Apoptosis of Hepatoma H_(22) Cells Induced by 5-Fluorouracil Combined with Fuzheng Yiliu Granule

作　　者：林如辉[1] 陈文列[1] 曹治云[1] 陈旭征 杜建[1] 

机构地区：[1]福建中医药大学中西医结合研究院,福建福州350108 [2]福建省中西医结合老年性疾病重点实验室,福建福州350108

出　　处：《福建中医药大学学报》2011年第3期28-30,共3页Journal of Fujian College of Traditional Chinese Medicine

基　　金：国家科技部国际科技合作项目(2008DFA32200)

摘　　要：目的观察氟尿嘧啶合扶正抑瘤颗粒诱导荷瘤小鼠肝癌细胞H22凋亡的超微结构。方法建立肝癌H22荷瘤小鼠动物模型。随机分为空白组、模型组、氟尿嘧啶组、氟尿嘧啶合扶正抑瘤颗粒4组,通过透射电镜观察4组肿瘤细胞的超微结构变化。结果氟尿嘧啶合扶正抑瘤颗粒组作用明显,其肝癌细胞核异染色质边聚成半月状,线粒体缩小明显,电子密度增加。结论氟尿嘧啶合扶正抑瘤颗粒能增强抗肿瘤作用,导致肝癌细胞的凋亡。Objective To observe the ultrastructure of apoptosis of hepatoma H22 cells in tumorbearing mice induced by 5-Fluorouracil （5-Fu） combined with Fuzheng Yiliu granule. Methods Tumorbearing mice models of H= Hepatoma were estabhshed and divided into control group, model group, 5-Fu group and 5-Fu combined with Fuzheng Yiliu granule group. The uhrastructural changes of tumor cells in four groups were observed by transmission electron microscope. Results Hepatoma H22 ceils in 5-Fu combined with Fuzheng Yiliu granule group had obvious changes, the mitochondrion was obviously reduced, the electron density was increased, and the heterochromatin around the caryotheca was clearly shown with a semilunar appearance. Conclusion 5-Fu combined with Fuzheng Yiliu granule can strengthen the antitumor effect, and promote the apoptosis of hepatoma Ha cells.

关 键 词：氟尿嘧啶 扶正抑瘤颗粒 肝癌细胞 凋亡 超微结构 

分 类 号：R96[医药卫生—药理学] R735.7[医药卫生—药学]