基于ISSR标记的辽宁4个东北林蛙种群遗传多样性分析  

Genetic Diversity of Rana dybowskii among Four Local Populations in Liaoning Province Based on ISSR Markers

作　　者：周瑜[1] 杨宝田[1] 陆宇燕[1] 巴山[1] 周正彦[1] 李丕鹏[1] 

机构地区：[1]辽宁省生物进化与多样性重点实验室,沈阳师范大学化学与生命科学学院,沈阳110034

出　　处：《四川动物》2011年第3期321-325,共5页Sichuan Journal of Zoology

基　　金：国家自然科学基金(No.30470206);辽宁省自然科学基金(No.20082198)

摘　　要：辽宁是东北林蛙Rana dybowskii主要分布地之一,种群数量巨大,其群体遗传多样性有待评估。本研究应用ISSR标记技术对东北林蛙4个种群105个样本进行研究,5个引物共获得44条清晰谱带,4个种群的多态位点率均大于75%,Nei s基因多样性为0·2851,Shannon信息指数为0·4476,显示了较高的遗传多样性。对遗传分化系数、Nei s遗传距离、AMOVA分子变异巢式方差分析和F-统计量等遗传参数的统计结果表明,辽宁东北林蛙种群间已经出现一定程度的遗传分化,分析认为,自然屏障(高山和平原等)以及栖息地片段化是其遗传分化形成的主要因素。Genetic diversity among 4 local populations of Rana dybowskii distributed in Liaoning province was analyzed using inter-simple sequence repeat （ISSR） markers.Five primers were used to amplify 105 individuals,and 44 bands were clearly tested.The populations of R.dybowskii showed a comparatively high genetic diversity.Among 4 local populations,the percentage of polymorphic loci was above 75%,Nei＇s gene diversity and Shannon index was 0.2851 and 0.4476,respectively.The result of Gst,Nei＇s genetic distance,analysis of molecular variance （AMOVA） and Fst showed that there were moderate genetic differentiation among local populations,and the genetic variation within populations was accounted for 78.54% of the total.It is suggested that the barriers of big mountains and the Liaohe plains played important roles in shaping the populations genetic differentiation of R.dybowskii.

关 键 词：东北林蛙 ISSR 遗传多样性 遗传分化 

分 类 号：Q958[生物学—动物学]