三叶鬼针草总黄酮的分离纯化工艺研究  

Study on the Purification Technology of Flavones in Bidens Pilosa L

作　　者：江海霞[1] 赵海[1] 

机构地区：[1]四川理工学院,四川自贡643000

出　　处：《广东化工》2011年第5期119-120,共2页Guangdong Chemical Industry

摘　　要：目的:研究三叶鬼针草中总黄酮类物质的聚酰胺分离纯化工艺。方法:采用分光光度法,以芦丁为标准品,通过用单因素试验优选分离工艺条件。结果:最佳分离条件为:上样浓度2.5 mg/mL,上样流速3 BV/h,用40%乙醇洗脱,洗脱速度为3 BV/h。结论:验证试验表明,该工艺稳定可靠。Objective： To study the purification technology with polyamide chromatographic of flavones in Bidens pilosa L.Methods： The optimum purification was studied by single factor experiment with spectrophotometry based on rutin as standard substance.Results： The optimum parameters of separation for flavones was follows： drug concentration was 2.5 mg/mL,absorping velocity of flow was 3 BV/h,the eluent was 40 % ethanol,and he eluting velocity of flow wais 3 BV/h.Conclusion： Validation experiment showed that the technology was steady and reliable.

关 键 词：三叶鬼针草 总黄酮 分离工艺 

分 类 号：TQ[化学工程]