体外诱导BMSCs向软骨分化的方法研究进展  

PROGRESS OF METHODS OF INDUCING BONE MARROW MESENCHYMAL STEM CELLS INTO CHONDROCYTES IN VITRO

作　　者：刘瑾春[1] 刘霞[1] 曹谊林[1] 

机构地区：[1]中国医学科学院北京协和医学院整形外科医院研究中心,北京100144

出　　处：《中国修复重建外科杂志》2011年第5期618-623,共6页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金青年科学基金资助项目(30801192);北京市自然科学基金资助项目(7102134);高等学校博士学科点专项科研基金(200800231078)~~

摘　　要：目的全面了解目前体外诱导BMSCs向软骨分化的方法,为软骨组织工程研究提供参考。方法广泛查阅近年来有关软骨组织工程中诱导BMSCs向软骨分化的文献,并进行综合分析。结果目前BMSCs诱导成软骨方法主要是添加外源性生长因子,其中TGF-β家族被公认为最重要的诱导和调节因子。其他重要的诱导方法包括添加多种化学因子、物理因素、转基因技术和微环境诱导等方法,但这些方法仍存在诱导效率低、诱导效果不稳定的问题。结论诱导方法的进展促进了BMSCs在软骨组织工程中的应用,建立更高效、简便、安全的诱导方法仍是软骨组织工程领域重要研究课题之一。Objective To review the research progress of the current methods of inducing bone marrow mesenchymal stem cells（BMSCs） to chondrogenic differentiation in vitro so as to provide references for researches in cartilage tissue engineering.Methods Various methods of inducing BMSCs differentiation into the chondrogenic lineage in vitro in recent years were extensively reviewed and analyzed.Results Adding exogenous growth factors is still the mainly method of inducing BMSCs differentiation into the chondrogenic lineage;among the members,transforming growth factor β（TGF-β） family is recognized as the most important chondrogenic induction factor.Other important inducing factors include various chemical factors,physical factors,transgenic methods,and the microenvironmental induction.But the problems of low inducing efficiency and unstable inducing effects still exist.Conclusion The progress of chondrogenic induction of BMSCs promotes its utilization in cartilage tissue engineering.Further researches are needed for establishing more efficient,simpler,and safer inducing methods

关 键 词：软骨组织工程 BMSCS 成软骨分化 生长因子 诱导 

分 类 号：R684[医药卫生—骨科学]