黄芪皂甙提高心脏保存效果的实验研究  

Experimental Studies on Improving Heart Preservation Effect of Astragalus Saponins

作　　者：储利胜 施雪筠[1] 席时芳[1] 孙承琳[1] 

机构地区：[1]北京中医药大学

出　　处：《中国中西医结合杂志》1999年第8期481-483,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家中医药管理局;国家教委博士点资助

摘　　要：目的：在改良的ＥｕｒｏＣｏｌｉｎｓ心脏保存液（ｍＥＣ液）中添加黄芪皂甙（ＡＳ），观察黄芪皂甙对心脏保存的作用。方法：将Ｗｉｓｔａｒ大鼠分成ｍＥＣ组和ＡＳ组。分别用ｍＥＣ液和含ＡＳ的ｍＥＣ液简单冷保存离体大鼠心脏６ｈ后，用Ｌａｎｇｅｎｄｏｒｆ离体鼠心模型再灌注３０ｍｉｎ，研究保存后的心功能及抗氧自由基损伤作用。结果：ＡＳ组与ｍＥＣ组比较，保存后心脏的心功能和冠脉流量明显好于ｍＥＣ组（Ｐ＜００５），心肌含水量显著降低（Ｐ＜００１），心肌细胞乳酸脱氢酶和肌酸激酶的漏出量显著减少（Ｐ＜００５和Ｐ＜００１），心肌细胞超氧化物歧化酶活性显著增高（Ｐ＜００１），而过氧化脂质含量明显降低（Ｐ＜００５）。结论：黄芪皂甙能提高ｍＥＣ液的心脏保存效果。Objective: To observe the effect of heart preservation of Astragalus saponins (AS) in modified Euro Collins solution (mEC) containing AS. Methods: Wistar rats were randomly divided into mEC and AS group. After preserving isolated rat hearts 6 hours by simple cold storage with mEC and mEC containing AS respectively, Langendorff′s isolated rat heart model was used to reperfuse for 30 minutes to study cardiac function after preservation and the effect of preventing oxygen free radical injury. Results: To compare AS group with mEC group, cardiac function and coronary flow of the hearts after preservation were better than mEC group (P<0 05), in AS group the water content of myocardium was decreased very significantly (P<0 01), myocardial lactate dehydrogenase and creatine phosphokinase release were reduced greatly (P<0 05 and P<0 01), myocardial superoxide dismutase activity was increased very significantly (P<0 01) and the content of lipid peroxide was decreased significantly (P<0 05). Conclusion: AS could improve the effect of heart preservation of mEC solution, its mechanism might be associated with the effect of negative contractile strength and preventing oxygen free radical injury.

关 键 词：黄芪皂甙 心脏保存 心脏保存液 药理 实验 

分 类 号：R282.710.3[医药卫生—中药学]