重组人组织因子途径抑制物-1对兔急性心肌梗死的作用  

The effect of recombinant human tissue factor pathway inhibitor-1 on Myocardial infarction in rabbits

作　　者：骆景光[1] 陈韵岱[2] 吕媛[2] 田峰[2] 王长华[2] 

机构地区：[1]首都医科大学附属复兴医院心内科,北京100038 [2]中国人民解放军总医院心内科

出　　处：《中华急诊医学杂志》2011年第5期507-510,共4页Chinese Journal of Emergency Medicine

基　　金：国家自然科学基金资助项目（30772289）

摘　　要：目的研究静脉应用重组人组织因子途径抑制物-1（recombinant tissue factor pathway inhibitor—1，rTFPI-1）对兔急性心肌梗死（acute myocardial infarction，AMI）冠状动脉再通后预防血栓形成，挽救存活心肌的作用。方法40只新西兰大白兔，结扎回旋支中段120min，再灌注60min。再灌注即刻随机分为对照组、大、中、小剂量rTFPI-1组，每组10只，活体Evan’S蓝染色测定缺血面积，离体心肌切片TIC染色测定心肌梗死面积。心肌缺血及梗死严重程度分别用缺血面积及梗死面积与左室壁心肌面积的比值表示。各组问基本情况、心肌缺血及梗死严重程度比较用完全随机设计单因素方差分析，均数两两比较采用LSD—t检验。结果大剂量rTFPI-1组心肌梗死严重程度较中、小剂量rTFPI-1组均明显减轻（P〈0．05或P〈0．01）；中剂量rTFPI-1组心肌梗死严重程度较小剂量rTFPI—1组和对照组明显减轻（P〈0．01）;小剂量rTFPI-1组和对照组比较差异无统计学意义（P〉0．05）。结论兔闭塞冠状动脉再灌注治疗时予rTFPI-1静点可显著减轻心肌梗死严重程度，挽救存活心肌。Objective To observe the function of recombinant human tissue factor pathway inhibitor- I （ rTFPI-1 ） in acute myocardial infarction in rabbit. Method Forty New Zealand White rabbits were sub- jected to coronary artery occlusion for 120 rain and followed by reperfusion for 60 rain, then they were ran- domly assigned into four groups： control group, large dose rTFPI-1 group, mod6rate dose rTFPI-1 group and low dose rTFPI-1 group （ n = 10/group）. The extent of ischemic area and the extent of myocardial infarction area were measured by Evan＇s blue stain and TFC stain, respectively. The degrees of infarction severity and ischemic severity were expressed as the ratios of the total left ventricular wall area. The degrees of infarction severity and ischemic severity in different groups were compared by using one-way ANOVA and then followed by LSD procedure. Results The degree of infarction severity in the larger dose rTFPI-1 group was signifi- cantly lessened than that in low dose RTFPI-1 group and control group （P 〈0. 001 ） , and than that in mod- erate dose rTFPI-1 group as well （ P 〈 0.05 ）. The degree of infarction severity in the moderate dose rTFPI- 1 group was significantly lessened than that in low dose rTFPI-1 group and control group （ P 〈 0. 001 ）. There was no significant difference in degree of infarction severity between low dose rTFPI-1 group and con- trol group （ P 〉 0.05 ）. Conclusions Human rTFPI-1 might decrease myocardial infarction severity and save the survival myocardial tissue.

关 键 词：急性心肌梗死 兔 组织因子途径抑制剂 凝血活酶(组织因子) 

分 类 号：R542.22[医药卫生—心血管疾病]