A型肉毒毒素注射引起大鼠前列腺增生模型细胞凋亡的研究  

Effects of Botulinum Toxin Type A on Cell Apoptosis in Rat Benign Prostatic Hyperplasia Model

作　　者：杨雄[1] 杨栋[1] 侯腾[1] 马彦 汪隆旺[1] 韩晓敏[1] 李兵[1] 陈朝晖[1] 

机构地区：[1]华中科技大学附属协和医院泌尿外科,武汉430022

出　　处：《临床泌尿外科杂志》2011年第5期389-391,共3页Journal of Clinical Urology

摘　　要：目的:探讨A型肉毒毒素注射后前列腺的组织形态学变化。方法:建立SD大鼠前列腺增生模型,模型大鼠前列腺内分别注射不同剂量的A型肉毒毒素后,TUNEL检测前列腺细胞的凋亡,免疫组织化学技术检测对照组与10U肉毒毒素注射组Bcl-2、Fas及Caspase-3的表达差异。结果:随着注射剂量的加大,大鼠前列腺体积及湿重出现明显下降,细胞凋亡增加。相比对照组,10U肉毒毒素注射组Bcl-2的表达下调而Fas及Caspase-3的表达上凋。结论:A型肉毒毒素能有效的缩小前列腺体积,引起前列腺细胞的凋亡。Objective：To investigate the morphological changes of rat prostate after the injection of Botulinum toxin type A. Methods：We established prostatic hyperplasia model of SD rats and injected different doses of botuli num toxin type A into the prostates of BPH rat model. Prostatic cells apoptosis was detected by TUNEL assay. The expression of Bcl-2.Fas, and Caspase-3 were evaluated as well. Results：In the botulinum toxin type A injection group, it was observed that the volume and wet weight of rat prostates were decreased, that cell apoptosis was increased. The expressions of Bcl-2, Fas, and Caspase 3 in 10U botulinum toxin injection group were differ ent compared to the control group. Conclusions：Botulinum toxin type A can diminish the volume and wet weight of rat prostate and induce apoptosis of prostatic cells.

关 键 词：肉毒毒素 前列腺增生 凋亡 

分 类 号：R971.92[医药卫生—药品]