云南保山和普洱地区带绦虫线粒体DNA基因编码核糖体RNA小亚基基因序列分析  

Analysis of the mitochondrial DNA-gene encoding ribosomalRNA small subunit gene sequence of Taenia cestode from Baoshan and Puer areas in Yunnan Province

作　　者：刘爱波[1] 杨毅梅[1] 

机构地区：[1]大理学院基础医学院寄生虫学教研室,去南省大理市671000

出　　处：《中华传染病杂志》2011年第4期236-238,共3页Chinese Journal of Infectious Diseases

基　　金：国家自然科学基金资助项目（30860252）

摘　　要：目的利用线粒体DNA基因编码核糖体RNA小亚基（mtDNA-12SrRNA）基因序列分析对采自云南保山、普洱地区的带绦虫标本进行鉴定。方法选取保山（7条，BSl～Bs7）、普洱（2条，PEl～PE2）带绦虫成虫节片，抽提基因组DNA，PCR扩增mtDNA-12SrRNA基因序列，并测序；结合GenBank中已知的猪带绦虫（ZD）、牛带绦虫（ND）、亚洲带绦虫（YZ）mtDNA-12SrRNA基因序列，经DNAMAN软件处理后构建同源树状图与系统发育树状图。结果带绦虫同源树与系统发育树状图显示，BSl、BS2、BS4、BS5与YZ的同源性最近（99％）。BS3、BS6、BS7、PEl、PE2与ND的同源性最近（99％）。结论云南保山存在牛带绦虫与亚洲带绦虫，普洱存在牛带绦虫，mtDNA一12SrRNA基因序列可用于三种带绦虫的分类研究。Objective To identify Taenia cestodes specimens collected from Baoshan and Puer regions of Yunnan Province by analyzing mitochondrial DNA gene encoding ribosomal RNA small subunit （mtDNA-12S rRNA） gene sequence. Methods The adult Taenia cestode samples were collected from Baoshan and Puer regions of Yunnan Province. The genomic DNA was extracted and mtDNA-12S rRAN gene was amplified by polymerase chain reaction （PCR）, then sequenced. Combined with the known mtDNA-12S rRNA gene sequence of Taenia soliurn, Taenia saginata, Taenia asiatica in GenBank, homology tree and phylogenetic tree were constructed by DNA MAN software. Results Taenia cestode homology tree and phylogenetic tree showed that gene sequences of BS1, BS2, BS4 and BS5 were most close to YZ with identity of 99% and those of BS3, BS6, BS7, PE1 and PE2 were most close to ND with identity of 99% Conclusions Taenia saginata and Taenia asiatica can be found in Baoshan area, while Taenia saginata can be found in Puer area. The gene sequence of mtDNA-12S rRNA can be used for clarifving the three tYPeS of Taenia cestode.

关 键 词：绦虫属 DNA 线粒体 RNA 核糖体 序列分析 RNA 

分 类 号：R346[医药卫生—基础医学]