异丙酚抑制长效酰胺类局麻药致大鼠中毒惊厥作用的比较  

Anticonvulsant Action of Propofol against Convulsion in Rats Induced by Long-Acting Amide Local Anesthetic

作　　者：韩倩[1] 张山[1] 乔玉敏[1] 王春平[1] 张志强[1] 

机构地区：[1]河北医科大学第二医院麻醉科,050000

出　　处：《天津医药》2011年第5期461-463,共3页Tianjin Medical Journal

基　　金：教育部"留学归国人员科研启动基金"项目(项目编号:教外司留[2005]383号);河北省卫生厅医学研究重点课题(项目编号:05054)

摘　　要：目的:比较异丙酚抑制左旋布比卡因、罗哌卡因和布比卡因所致惊厥的作用。方法:64只Wistar大鼠随机分为对照组(C组)、左旋布比卡因组(L组)、罗哌卡因组(R组)和布比卡因组(B组),4组内再分为实验(EG)和异丙酚处理(PG)2个亚组(n=8)。L组、R组和B组分别经尾静脉泵入0.75%相应局麻药,速度均为2mg·kg-1·min-1,PG各亚组在出现惊厥反应后静脉注射2mg/kg异丙酚。C(EG)组泵入生理盐水,C(PG)组泵入生理盐水后静脉注射异丙酚。惊厥2h后,测定海马CA1区c-fos阳性细胞数、NO水平和一氧化氮合酶(NOS)活性表达。结果:除C组外各组均有惊厥发生。异丙酚处理组在给予异丙酚后很快抑制惊厥。与C(EG)组比较,L(EG)、R(EG)及B(EG)组海马CA1区c-fos阳性细胞数、NO含量以及NOS活性表达均显著增加(P<0.01)。L(PG)、R(PG)和B(PG)组海马CA1区c-fos阳性细胞数、NO含量以及NOS活性表达较对应的L(EG)、R(EG)及B(EG)组均明显减少(P<0.01)。L组与R组较B组c-fos阳性细胞数、NO水平和NOS活性表达的K值明显减小(P<0.01)。结论:异丙酚能在一定程度上抑制左旋布比卡因、罗哌卡因和布比卡因引起的惊厥,且对布比卡因致惊厥的抑制作用强于左旋布比卡因和罗哌卡因。Objective：To compare the effect of propofol on the experimental convulsions in rats induced by levobupivacaine,ropivacaine and bupivacaine.Methods：Sixty-four male Wistar rats were randomly divided into control group（group C）,levobupivacaine group（group L）,ropivacaine group（group R） and bupivacaine group（group B）.Each group was subdivided into two sub-groups（n=8）,experimental group（EG） and propofol treatment group（PG）.Rats were infused with 0.75% levobupivacaine,0.75%ropivacain or 0.75% bupivacaine at a same rate of 2 mg·kg-1· min-1 in group L（EG）,group R（EG） and group B（EG） respectively.Propofol was infused 2 mg/kg i.v.when seizure developed in group L（PG）,group R（PG） and group B（PG）.Normal saline was infused in group C（EG）,and propofol was given after normal saline infusion in group C（PG）.The values of c-fos expression,nitric oxide（NO） and nitric oxide synthase（NOS） in hippocampus CA1 section were measured 2 hours after convulsion.Results：Convulsion appeared in rats of every group except group C.Rats which developed convulsion could quickly be suppressed after propofol was infused i.v.in group PG.Compared with group C（EG）,the values of c-fos expression,the concentration of NO and the activity of NOS in hippocampus CA1 were significantly higher in group L（EG）,group R（EG）and group B（EG）（P 0.01）.Compared with group L（EG）,group R（EG）and group B（EG） ,the expression of c-fos,the concentration of NO and the activity of NOS in hippocampus were significantly lower in group L（PG）,group R（PG）and group B（PG）（P 0.01）.The K-values of the expression of c-fos,the concentration of NO and the activity of NOS were significantly less in group L and group R than those of group B（P 0.01）.Conclusions：Propofol may inhibit the convulsion induced by levobupivacaine,ropivacaine and bupivacaine to some extent.From the changes of the expression of c-fos,the concentration of NO and the activity of NOS,propofol may

关 键 词：二异丙酚 麻醉药 局部 发作 原癌基因蛋白质C-FOS 海马 一氧化氮 一氧化氮合酶 疾病模型 动物 

分 类 号：R971.2[医药卫生—药品]