EDTA-Na_2脱钙模拟骨质疏松及打孔对骨密度的变化影响  

Effect of EDTA-Na_2 decalcification to simulate osteoporosis and drilling on the alteration of bone mineral density

作　　者：程自申[1] 张智海[2] 刘莉[3] 尹磊[4] 李鹏[1] 王岩[1] 肖嵩华[1] 张永刚[1] 张雪松[1] 毛克亚[1] 

机构地区：[1]解放军总医院骨科,北京100853 [2]北京航空工业中心医院脊柱外科 [3]河北保定第一中心医院妇产科 [4]河北保定解放军252医院神经外科

出　　处：《中国骨质疏松杂志》2011年第4期296-299,共4页Chinese Journal of Osteoporosis

基　　金：国家自然科学基金(编号:39670731);国家自然科学基金(编号:50830102);国家863计划(编号:2009AA02Z405)

摘　　要：目的观察利用乙二胺四乙酸二钠盐(EDTA-Na2)椎体脱钙骨密度的变化,以及打孔对椎体骨密度的影响,探讨一种制作骨质疏松模型的方法。方法取98个新鲜猪单胸腰椎椎体,随机分成14小组,每组7个标本,1～7组全部经椎弓根打孔,记A大组,8～14组不行椎弓根打孔,记为B大组,然后把所有标本侵入0.4916mmol/L的EDTA-Na2脱钙液体中,分别在0、5、7、9、11、13、15天取出A大组与B大组各一小组行骨密度测试及每小组取一个标本大体剖开。观察脱钙时间与A、B两组骨密度变化及两组差异关系。结果 A、B随着脱钙时间的延长,大体观察可见各组标本骨小梁逐渐变细、间隙增宽,骨密度检测结果显示逐渐减低,并且各组脱钙前后骨密度变化均有明显的差异性(P=0.0000)。A与B大组在骨密度的变化无明显的差异(P>0.05)。结论采用0.4916mmol/L EDTA-Na2脱钙0～15天可模拟轻、中、重度骨质疏松,打孔对骨密度变化并没有影响。Objective To observe the alteration of bone mineral density （BMD） of the vertebra body using ethylenediaminetetraacetic acid disodium salt （ EDTA-Na2 ） decalcification, and the effect of drilling on BMD of the vertebra body. Methods Ninety-eight single fresh porcine thoracic or lumbar vertebral bodies were randomly divided into 14 groups, with 7 specimens in each group. The specimens in group 1-7 were drilled two holes in the vertebral pediele, marked Group A. The specimens in group 8-14 were not drilled, marked Group B. All the specimens were dipped in 0. 4916mmol/L of EDTA-Na2 decalcification solution. One specimen in every group of Group A and B were collected for BMD examination and for sectioning, on day 0, 5, 7, 9, 11, 13, and 15, respectively. The relationship between the alteration of BMD and the decalcification time in Group A and B group was observed. Results Following the extension of decalcification time, gross observation showed that the trabecular bone was getting thinner, and the gap was getting wider in all specimens of both Group A and B. BMD testing showed that BMD appeared a trend of gradual reduction in all specimens, and the alteration of BMD in specimens of each group was significant （P =0. 0000） before and after the decalcification. The alteration of BMD between Group A and B was not significant （ P 〉 0. 05 ）. Conclusion Decalcification using 0. 4916mmol/L EDTA-Na2 could simulate miner, moderate, and severe osteoporosis. No effect of drilling on BMD was found.

关 键 词：骨质疏松 EDTA—Na2 原位脱钙 骨密度 

分 类 号：R681[医药卫生—骨科学]