5-脂氧酶表达与慢性铝过负荷致大鼠脑损伤的关系  

Experimental study on relationship between 5-LO expression and brain damage induced by chronic aluminum overload in rats

作　　者：张鹏[1] 杨俊卿 苏强[2] 

机构地区：[1]遂宁市中心医院,四川遂宁629000 [2]重庆医科大学药学院药理教研室

出　　处：《中国老年学杂志》2011年第9期1558-1560,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(30672211)

摘　　要：目的初步研究5-脂氧酶(LO)表达与慢性铝过负荷致大鼠脑损伤的关系。方法灌胃给予大鼠葡萄糖酸铝(A l3+200 mg/kg),1次/d,5 d/w,持续20 w,建立慢性铝过负荷致大鼠脑损伤、神经元退变动物模型;5-LO抑制剂咖啡酸(30 mg/kg、10 mg/kg)分别在每次大鼠铝给予1 h后灌胃。以大鼠空间学习记忆能力改变、海马病理形态学变化以及脑组织5-LO蛋白和5-LO mRNA表达变化为观察指标。结果慢性铝过负荷明显导致大鼠空间学习记忆能力下降、海马神经元损伤、脑组织5-LO蛋白和5-LO mRNA表达明显增加。给予咖啡酸能明显改善慢性铝过负荷大鼠的学习记忆能力障碍,明显减轻铝过负荷大鼠海马神经细胞的损伤,明显阻遏铝慢性过负荷诱导的5-LO蛋白和5-LO mRNA表达增加。结论 5-LO的过表达与慢性铝过负荷大鼠脑损伤、神经元退变之间存在着紧密的联系。Objective To explore the relationship between 5-LO expression and brain damage induced by chronic aluminum overload in rats.Methods The brain injury model of rats was established via intragastric administration of aluminum gluconate（element aluminum 200 mg/kg） once a day,5 d/w for 20 weeks.The 5-LO inhibitor caffeic acid（30,10 mg/kg） was intragastrically administered 1 h after aluminum administration.Pathomorphological changes of hippocampal neuron and the spatial learning and memory function were observed.The 5-LO mRNA expression in hippocampus was detected by RT-PCR and the 5-LO protein in hippocampus was detected by Western-blot.Results Chronic aluminum overload could markedly induce spatial learning and memory function impairment and hippocampal neuronal injury in rats.The 5-LO mRNA expression and 5-LO protein significantly increased in chronic aluminum overload rats.The administration of the caffeic acid could significantly prevent rats from learning and memory function impairments and hippocampus neuronal death.Caffeic acid also obviously decreased 5-LO mRNA expression and 5-LO protein of chronic aluminum overload rats.Conclusions There is a close connection between over-expression of 5-LO and neuron damage induced by chronic aluminum overload.And administration of selective inhibitor of 5-LO can prevent the rat brain from damage induced by chronic aluminum overload.

关 键 词：慢性铝过负荷 5-LOmRNA 5-LO蛋白 脑损伤 联系 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]