电针“内关”穴预处理对缺血再灌注大鼠心肌阿片受体基因表达的影响  

Effects of electroacupuncture preconditioning at "Neiguan"(PC 6) on gene expression of myocar-dial opioid receptors in rats with myocardial ischemia-reperfusion injury

作　　者：李万山 钟敏 杨进辉 招伟贤 

机构地区：[1]广州中医药大学第二附属医院,广东广州510120 [2]广州市越秀区正骨医院

出　　处：《中国针灸》2011年第5期441-445,共5页Chinese Acupuncture & Moxibustion

基　　金：广东省中医药局资助项目:2008115

摘　　要：目的:探讨"内关"穴预处理对心脏保护的效应及作用机制。方法:健康雄性Wistar大鼠48只,随机分为假手术组(N组)、心肌缺血模型组(M组)、电针组(E组)。M组采用结扎左冠状动脉前降支(LAD)30 min、再灌注120 min的方法制备心肌缺血再灌注模型;N组只开胸穿线,不结扎LAD;E组在缺血前3 d每天给予双"内关"穴电针刺激20 min。用TTC染色的方法测定大鼠心梗面积;用生化方法测定血中肌酸激酶同工酶(CK-MB)及乳酸脱氢酶(LDH)含量;光镜下观察心肌形态学改变;应用实时荧光定量RT-PCR法测定心肌δ阿片受体(DOR)和κ阿片受体(KOR)mRNA的表达。结果:E组较M组心肌梗死面积明显缩小(P<0.05)。M组CK-MB和LDH水平分别为(980±92)U/L和(2 743±124)U/L,均较N组的(312±41)U/L和(530±56)U/L显著升高(均P<0.01),E组的CK-MB及LDH水平分别为(572±70)U/L和(1819±97)U/L,均较M组明显降低(均P<0.01)。DOR和KOR表达在M组与N组间无明显差别(均P>0.05),E组DOR表达较M组和N组显著增加(均P<0.01),KOR表达在3组间差异无统计学意义(均P>0.05)。结论:电针"内关"穴预处理可通过上调心肌δ阿片受体基因表达对缺血再灌注心肌产生保护作用。Objective To explore the protective effects of electroacupuncture preconditioning at ＂Neiguan＂ （PC 6） on myocardial ischemia reperfusion injury （MIRI） and the mechanism. Methods Forty-eight male Wistar rats were randomly divided into a sham operation group （group N）, a MIRI group （group M） and an electroacupunc-ture group （group E）. The MIRI model was generated by ligating the left anterior descending artery （LAD） for 30 min followed by reperfusion for 120 min. The partition sutures in group N were passed under the LAD without ligation. The rats in group E were pretreated with electroacupuncture applied at bilateral ＂Neiguan＂ （PC 6） for 20 min once a day on 3 consecutive days before ischemia. The area at risk and infarct size and the serum levels of CK-MB and LDH were measured. The morphological changes were observed by light microscopy. The mRNA expressions of δ and κ opioid receptor （DOR and KOR） were determined by real-time RT-PCR. Results The myocardial infarct size in group E was significant smaller than that in group M （P〈0.05）. The contents of CK-MB and LDH were （980±92）U/L and （2 743±124）U/L in group M which were significant higher than （312±41） U/L and （530±56）U/L in group N （both P〈0.01） and （572±70）U/L and （1 819±97）U/L in group E （both P〈0.01）. The mRNA expressions of DOR and KOR had no significant difference between group M and N （both P〉0. 05）, while the DOR expression in group E was significant higher than that in group M and N （both P〈 0. 01）. There was no significant difference in KOR expression among three groups （all P〉0.05）. Conclusion The cardiac protective effects of electroacupuncture preconditioning at ＂Neiguan＂ （PC 6） on MIRI may be mediated by increasing expression of δ opioid receptor in myocardium.

关 键 词：电针 穴 内关 缺血再灌注损伤 心肌 受体 阿片 

分 类 号：R246[医药卫生—针灸推拿学]