α-硫辛酸对神经突触生长的促进作用  

α-Lipoic acid promotes the neurite outgrowth

作　　者：李竞进[1] 王竹瑶[1] 王晓辉[1] 刘莉[2] 丁正年[1] 

机构地区：[1]南京医科大学第一附属医院麻醉科,210029 [2]南京医科大学第一附属医院老年医学科,210029

出　　处：《江苏医药》2011年第9期993-995,F0003,共4页Jiangsu Medical Journal

基　　金：国家自然科学基金(30972856;81071752;81071067);江苏省自然科学基金(BK2008467);江苏省六大人才高峰(07-B-041)

摘　　要：目的探讨α-硫辛酸（LA）对神经突触生长的影响。方法 （1）N2a细胞接受作用浓度为100、300和500μM的LA刺激,分别于刺激后24～60 h在显微镜下观察突触生长情况。未接受LA刺激的细胞作为对照组。每组4份。（2）大鼠原代海马神经元接受500μM的LA刺激,分别于刺激后24和36 h在显微镜下观察突触生长情况。未接受LA刺激的细胞作为对照组。每组4份。结果 （1）LA作用于N2a细胞24 h后,各组突触生长细胞百分比分别为：对照组（1.85±0.6）%、100μM组（5.3±4.1）%、300μM组（7.8±4.3）%、500μM组（12.5±3.3）%;与对照组比较,只有500μM LA组的突触生长细胞百分比显著增加（P〈0.01）。LA作用60 h后,各组突触生长细胞百分比分别为：对照组（12.5±3.7）%、100μM组（23.2±6.2）%、300μM组（35.0±4.9）%、500μM组（67.6±4.3）%;三个LA处理组的突触细胞百分比均比对照组显著增加（P〈0.01或P〈0.05）。（2）LA作用于大鼠原代海马神经元后24、36 h,突触长度较对照组分别增加了22.9%、30.5%（P〈0.01）。结论 LA对体外培养神经元具有显著促进神经元突触生长的生物学作用。Objective To investigate the role of α-lipoic acid（LA） in neurite outgrowth.Methods（1）Mouse neuroblastoma N2a cells were treated with LA at different concentrations（100,300 and 500 μM） for different periods（24 h and 60 h） and untreated cells served as the control with 4 samples in each group.Neurite outgrowth was examined under microscope.（2）Rat primary hippocampal neurons were treated with 500 μM LA for 24 h and 36 h,respectively,and untreated cells served as the control with 4 samples in each group.Neurite outgrowth was examined under microscope.Results（1）After treated with LA for 24 h,the percentages of neurite-bearing cells were（1.85±0.6）%,（5.3±4.1）%,（7.8±4.3）% and（12.5±3.3）% for groups of control,100,300 and 500 μM LA,respectively,which of 500 μM LA were greater than those of control group（P0.01）.After treated with LA for 60 h,the percentages of neurite-bearing cells were （12.5±3.7）%,（23.2±6.2）%,（35.0±4.9）% and（67.6±4.3）% for groups of control,100,300 and 500 μM LA,respectively,which of LA-treated groups were greater than those of control group（P0.01 or P0.05）.（2）Compared with the control,the neurite length was significantly increased by 22.9% and 30.5% in rat primary hippocampal neurons treated with LA for 24 and 36 h,respectively（P0.01）.Conclusion LA promotes neurite outgrowth in vitro.

关 键 词：神经突触 Α-硫辛酸 

分 类 号：R614[医药卫生—麻醉学]