新型多孔可吸收骨替代材料修复兔股骨缺损的实验研究  

New porous resorbable bone graft substitute to repair femoral defects in rabbits

作　　者：刘善宇[1] 王钢[1] 李岗[1] 贾谊[1] 

机构地区：[1]南方医科大学南方医院创伤骨科,广州510515

出　　处：《中华创伤骨科杂志》2011年第5期437-442,共6页Chinese Journal of Orthopaedic Trauma

摘　　要：目的 探讨新型多孔块状可吸收生物活性玻璃骨替代材料的骨缺损修复能力和生物机容性。方法 选取30只健康成年新四兰大白兔，建立双侧股骨髁直径为6mm、深10～12mm的骨缺坝模型，根据骨缺损中植入的材料不同随机分为3组，每组10只20侧：实验组（A组）植人多孔块状叮吸收乍物活性玻璃骨替代材料，材料对照组（B组）植人常川非多孔生物活性玻璃骨替代材料，夺一对照组（c组）不植入任何材料．．术后6、12周取材，通过大体观察、x线片、显做CT和病理组织切片检测评价新刑多孔块状可吸收牛物活性玻璃骨替代材料的成骨能力和生物降解性能。结果植入后6用A、B组可见新生骨逐渐向材料内部生长，A组新生骨的数城明显多于B组。12刷时A组卡，f人材料几乎完仓降解，骨缺损完全修复，成熟的骨小梁塑形改造；B组植骨中心区仍有较多材料尚未完全降解；C组骨缺损末愈合。组织切片定量分析显示：6周时A、B、C组新生骨占骨缺损的面积比平均分别为0．30±0．02、0．17±0．03、0．06±0．0l，差异有统计学意义（F=374．202，P〈0．001），组间两两比较苁异均有统计学意义（P〈0．05）；12间时A、B、c组新牛骨占骨缺损的面积比平均分别为0．53±0．05、0．39±0．05、0．07±0．0l，差异有统计学意义（F：317．243，P〈0．001），组间两两比较差异均有统计学意义（P〈0．05）。，A组与B组6、12周时残留材料占骨缺损面积比差异均有统计学意义（P〈0．05）。结论新型多孔块状可吸收生物活性玻璃骨替代材料闪具有良好的一维结构而表现出更好的成骨能力和生物降解性能。Objective To test the osteogenetie ability and bioeompatibility of a new type of porous morsel blocks of resorbable bioglass bone graft substitute. Methods Thirty heahby New Zealand aduh white rabbits were selected to establish bone defect models anti randomly assigned into 3 even groups （with 20 hind legs in each group） for 3 methods of repairing the defects. The 6 mm x 10 to 12 mm defects were made at the bilateral femoral condyles of each rabbit. In group A, a porous mnrsel block of resorbable bioactive glass bone substitute was implanted; in group B, commonly used bioaetive glass was implanted, in group C, nothing was implanted. Specimens were collected at 6 and 12 weeks after operation to evaluate the biodegradation and osteogenic properties by gross observation, radiography, Micrn-CT and histopathological examinations. Results No local adverse reaction was observed in each group. At 6 weeks, gradual growth of the new bone was observed inside the implants in groups A and B. The amount of new bone in group A was significantly higher than in group B. In group A, the porous morsel material was almost completely degraded, bone defects were completely repaired, and bone trabecula moulding reconstruction was eompleted. In group B, a great amount of material was not completely degraded in the central area of the graft, while bone defects were not healed in the group C. The percentages of new bone area were 0.30 ＋ 0.02 in group A, 0. 17 ＋ 0.03 in group B anti0.06 ＋ 0.01 in group C, with significant dift^nces （F=374.202, P 〈0.001） . At 12 weeks, the percentages of new bone area were O. 53 ±0. 05 in group A, O. 39 ± 0. 05 in group B and 0.07 ± O. O1 in group C, with significant differences （ F =317. 243, P 〈 O. 001 ） . Conclusion New porous morsel blocks of resorbable bioglass bone graft substitute show superior osteogenetic properties and biodegradability because of their good three dimensional structure.

关 键 词：生物相容性材料 股骨 材料试验 兔 骨缺损 

分 类 号：R318.08[医药卫生—生物医学工程]