低强度牵张力对大鼠尾椎间盘炎症因子表达的影响  

Effects of tension stress on the expression of inflammatory cytokines in intervertebral disc in rat

作　　者：韩超[1] 马信龙[1,2] 马剑雄[1] 王涛[1] 孙晓雷[2] 田鹏[2] 臧加成[1] 

机构地区：[1]天津医科大学总医院骨科,300052 [2]天津市天津医院

出　　处：《中华骨科杂志》2011年第5期556-562,共7页Chinese Journal of Orthopaedics

基　　金：天津市卫生局科技基金（09KR08）;天津市卫生局重点学科基金项目（10KG111）;天津市科委应用基础与前沿技术研究计划重点项目（09JCZDJC19600）

摘　　要：目的 探讨牵张力对大鼠尾椎间盘中白细胞介素17B（interleukin17B,IL-17B）及诱导型一氧化氮合酶（inducible nitric oxide synthase,iNOS）mRNA表达的影响.方法 64只雌性SD大鼠随机分为四组：正常对照组（S）、悬吊组（TS）、悬吊＋针刺组（TSNP）及单纯针刺组（SNP）,每组16只.通过大鼠尾吊装置使TS、TSNP组大鼠后肢悬空,对其尾椎间盘施加牵张力;通过对TSNP、SNP组大鼠第七尾椎间盘进行针刺,制造椎间盘周围炎性环境.造模4周后,取各组大鼠的第七尾椎间盘进行大体标本观察、组织学观察以及荧光定量逆转录聚合酶链式反应分析.结果 大体及组织学观察发现TS、SNP及TSNP组中纤维环和髓核的形态结构较S组均出现不同程度的退行性改变.荧光定量逆转录聚合酶链式反应结果显示：与S组相比,SNP组和TSNP组IL-17B及iNOS的mRNA表达水平均显著升高,其表达量的比值（即2-△△Q值）分别为：22.49、12.99和14.98、8.12,这两组的表达水平与S组比较差异均有统计学意义.而TS组中IL17B及iNOS的表达则仅轻度升高,2-△△Q值仅为1.22和1.49,与S组比较差异无统计学意义.TSNP组和SNP组比较发现,IL17B及iNOS在TSNP组中的表达远低于SNP组,其表达量的差值分别为-9.50和-6.86.结论 相对低水平的牵张力能够在抗炎反应中起到积极作用,这为临床应用牵引疗法治疗椎间盘源性疼痛提供了一定的理论依据.Objective To investigate the effect of tension stress on the changes of mRNA expression of interleukin-17B （IL-17） and inducible nitric oxide synthase （iNOS） in rat discs.Methods Sixty-four female Sprague-Dawley rats were randomly divided into 4 groups：sham （S）,tail-suspended （TS）,tail-suspended with needle puncture （TSNP） and single needle puncture （SNP） groups.Tail-suspension device made rats of TS group and TSNP group hindlimb suspended,which exerted tension force on the coccygeal vertebrae disc;and aseptic needle puncture on the tail disc between the seventh and eighth coccygeal vertebrae （Co7/Co8）of TSNP,SNP group induced the inflammatory environment.The discs were harvested 4 weeks later.Measurements included gross observation,histological observation and real-time quantitative reverse transcription-polymerase chain reaction （RT-qPCR） analysis.Results Gross and histological examination found that varying degrees of degenerative changes in the annulus and nucleus in TS,SNP and TSNP groups,but not in the S group.RT-qPCR results showed that the mRNA of IL-17B and iNOS were significantly higher in the TSNP and SNP groups than that in the S group.The values of 2-△△Q were 12.99 and 8.12 in TSNP groups,and the 2-△△Q values of SNP group demonstrated 22.49 and 14.98 compared to S groups.Sole application of tension stress brought by the tail-suspension slightly enhanced the expression of IL-17B and iNOS,the values of 2-△△Q were 1.22 and 1.49,which didn＇t reach a statistically significant change.Compared with SNP group,the mRNA expression of IL-17B and iNOS in TSNP groups were shapely decreased,the values of 2-△△Q were-9.50 and -6.86 respectively.Conclusion Relatively low magnitude tension stress might play a key role in anti-inflammatory process and the relief of discogenic pain.

关 键 词：应力 物理 白细胞介素17 腰痛 

分 类 号：R681.53[医药卫生—骨科学]