猪TCTP基因的表达规律及其对脂肪细胞分化的影响  

Expression of Porcine TCTP and Its Effect on Differentiation of Adipocytes

作　　者：李新建[1,2] 杨浩[1] 程佳[1] 宋子仪[1] 杨公社[1] 

机构地区：[1]西北农林科技大学动物脂肪沉积与肌肉发育实验室,杨凌712100 [2]河南农业大学畜牧兽医工程学院,郑州450002

出　　处：《中国生物化学与分子生物学报》2011年第5期444-451,共8页Chinese Journal of Biochemistry and Molecular Biology

基　　金：转基因生物新品种培育重大专项(No.2009ZX08009-157B)~~

摘　　要：翻译控制肿瘤蛋白(TCTP,translationally controlled tumor protein)是一类广泛存在各种生物、序列高度保守的蛋白,最初认为TCTP是一类生长相关蛋白,近年研究发现TCTP可能具有非常重要的生物学功能.本研究通过高通量测序(Solexa)技术、实时定量PCR(RT-qPCR)对瘦肉型和脂肪型猪不同生长阶段脂肪组织、脂肪细胞中TCTP的表达规律进行了研究,采用siRNA技术,沉默TCTP,研究了其对脂肪细胞分化的影响.结果表明:TCTP在瘦肉型猪脂肪组织中的mRNA表达量显著高于脂肪型猪(P<0.01);在不同日龄猪脂肪组织中,TCTP的mRNA表达量随着日龄增长而降低;在不同组织中的检测结果发现,TCTP在心、肝、肾、肌肉和脂肪中有较高的表达,肺和脾中表达量较低;TCTP的mRNA表达量在猪前体脂肪细胞增殖过程中逐渐增高,在分化阶段逐渐下降;沉默TCTP促进了脂肪细胞的分化,引起了PPARγ、C/EBPα、SREBP-1c的显著升高(P<0.01).以上研究发现,TCTP在脂肪沉积过程中可能具有抑制作用,为进一步研究肥胖关键基因调控机制提供科学依据.The translationally controlled tumor protein（TCTP） expressed abundantly in a wide range of organisms from both the animal and plant kingdom,which was initially described as a growth-related protein.However,the studies showed that TCTP has a lot of biological functions.The expression of TCTP in fat tissues of different genotype pigs and in different growth periods were explored by high-throughout sequencing（Solexa） and real-time quantity PCR（RT-qPCR）,and its effect on differentiation of adipocytes was explored by siRNA.The results showed that expression level of TCTP was higher in the fat tissues of lean-type pig than that of obese-type.Expression level of TCTP in the porcine fat tissue and adipocyte was influenced by ages,and showed a higher expression in heart,liver,kidney,muscle and fat,but lower in spleen and lung.Silencing TCTP by siRNA promoted the adipocytes to differentiate and significantly increased expression levels of PPARγ,C /EBPα and SREBP-1c.TCTP may inhibit adipogenesis by this study which will provide new theoretical reference for fat deposition control.

关 键 词：翻译控制肿瘤蛋白 高通量测序 实时定量PCR 猪脂肪组织 SIRNA干扰 分化 

分 类 号：S811.3[农业科学—畜牧学] Q81[农业科学—畜牧兽医]