青蒿素对大鼠子宫内膜异位症的抑制作用及机理探讨  

Inhibition Effect and Mechanism of Artemisnin on Surgically Induced Endometriosis

作　　者：王雪[1] 方堃[2] 王晓茜[1] 王红静[1] 贾西标[1] 杨凌云[1] 

机构地区：[1]四川大学华西第二医院妇产科,成都610041 [2]贵州省人民医院妇科,贵阳550000

出　　处：《四川大学学报（医学版）》2011年第3期364-368,共5页Journal of Sichuan University(Medical Sciences)

摘　　要：目的探讨青蒿素对大鼠子宫内膜异位症模型的异位内膜是否具有抑制作用,以及其抑制作用与细胞凋亡和血管生成之间的关系。方法通过手术方法建立大鼠子宫内膜异位症模型,4周后测量异位内膜体积,将造模成功的大鼠(n=40)分为4组:青蒿素高剂量组〔300 mg/(kg.d)〕、青蒿素低剂量组〔150 mg/(kg.d)〕、丹那唑组〔160 mg/(kg.d)〕和溶剂对照组。每日经灌胃给药,持续4周后处死大鼠,比较各组病灶体积及组织学变化,免疫组化法检测各样本的凋亡指数、Bcl-2和微血管密度。结果与溶剂对照组相比,青蒿素高剂量组、青蒿素低剂量组和丹那唑组异位内膜的体积减少(P均<0.05),但3个治疗组的异位内膜体积差异无统计学意义。与溶剂对照组相比,3个治疗组异位内膜组织的凋亡指数增高,Bcl-2表达和微血管密度降低(P均<0.05);除微血管密度外,青蒿素组凋亡指数较丹那唑组更高,Bcl-2表达较丹那唑组更低(P均<0.05),且呈剂量依赖关系。各组大鼠未见明显的毒副反应。结论青蒿素对大鼠子宫内膜异位症模型中异位内膜的发生及发展有抑制作用,其抑制效应可能与促进细胞的凋亡和抑制血管生成有关。Objective To evaluate the inhibitory effect of artemisnin on surgically induced endometriosis in rat model and the possible mechanism related to cellular apoptosis and microvascular angiogenesis.Methods Surgically induced endometriosis model was established with female rats,and then the rats were divided into four groups： high dose artemisinin（300 mg/（kg·d））,low dose artemisinin（150 mg/（kg·d））,danazol（160 mg/（kg·d）） and solvent control group After daily administration of the above agents for 4 weeks,the rats were sacrificed,then the implant size of ectopic endometrium was measured and appotosis index（AI）,Bcl-2 and MVD in ectopic endometrium were evaluated with S-P immunohistochemistry.Results Compared with solvent control,both artemisinin（high and low quality） and danazol decrease the size of implants significantly（P〈0.05）,and there was no significant difference among the three treatment groups.AI of the three treatment groups increased significantly,while Bcl-2 and MVD decreased significantly（P〈0.05）.AI of both artemisinin groups were significantly higher than that of danazol group,but Bcl-2 level was lower.Conclusion Artemisnin inhibits surgically induced endometriosis in rats,and the possible mechanism may be related to stimulatation of cellular apoptosis and inhibition of angiogenesis.

关 键 词：青蒿素 丹那唑 子宫内膜异位症 

分 类 号：R285.5[医药卫生—中药学]