急性肝衰竭模型中医证型探讨  

Discussion of Chinese Syndrome Typing in Acute Hepatic Failure Model

作　　者：张金良[1,2] 曾辉[1] 王宪波[1] 

机构地区：[1]首都医科大学附属北京地坛医院,北京100015 [2]武警内蒙古总队医院呼和浩特,010040

出　　处：《中国中西医结合杂志》2011年第5期659-662,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81072766);北京市自然科学基金资助项目(No.7112066)

摘　　要：目的应用小鼠急性肝衰竭模型,筛选有效方剂,以方测证探讨该模型的中医证型。方法采用腹腔注射脂多糖(lipopolysaccharides,LPS)/D-氨基半乳糖胺(D-galactosamine,D-GalN)建立小鼠急性肝衰竭模型,给予茵陈蒿汤、黄连解毒汤、补中益气汤、犀角地黄汤灌胃干预。观察动物的行为及生存率,检测肝功能,评价肝组织病理损伤。结果所测经典方剂中,犀角地黄汤可使小鼠急性肝衰竭模型的生存率由10%提高到60%。造模5h后,犀角地黄汤组ALT为(183.95±52.00)U/L、AST为(235.70±34.03)U/L低于模型对照组[ALT:(213.32±71.93)U/L;AST:(299.48±70.56)U/L],但差异无统计学意义(P>0.05);犀角地黄汤可以明显改善肝脏的损伤。结论犀角地黄汤是治疗LPS/D-GalN急性肝衰竭模型的有效方剂,按照以方测证原则,LPS/D-GalN小鼠急性肝衰竭模型以热毒血瘀证为主。Objective To study Chinese syndrome typing of acute hepatic failure （AHF） mice model by screening effective formulae. Methods Lipoplysaccharides （ LPS）/D-galactosamine （D-GaiN） was intraperitoneally injected to mice to establish the AHF mice model. Yinchenhao Decoction, Huanglian Jiedu Decoction, Buzhong Yiqi Decoction, and Xijiao Dihuang Decoction were administered to model mice respectively by gastro- gavage. The behavior and the survival rate were monitored. The liver function and pathological changes of liver tissues were detected. Results In all the tested classic recipes, the survival rate was elevated from 10% to 60% by administration of Xijiao Dihuang Decoction. Five h after modeling, the serum alanine aminotransferase （ALT） level was （183.95±52.00） U/L, and aspartate aminotransferase CAST）（235.70 4±34.03） U/L in Xijiao Di- huang Decoction Group, lower than those of the model control group, but with insignificant difference （ALT. 213.32±71.93 U/L; AST： 299.48±70.56 U/L, both P〉0.05）. Xijiao Dihuang Decoction could obviously alle- viate the liver injury. Conclusions Xijiao Dihuang Decoction was an effective formula for LPS/D-GalN induced AHF model. According to syndrome typing through formula effect, heat toxin and blood stasis syndrome dominated in the LPS/D-GalN induced AHF mice model.

关 键 词：急性肝衰竭 脂多糖 D-氨基半乳糖胺 犀角地黄汤 热毒血瘀证 

分 类 号：R259[医药卫生—中西医结合]