白毛藤总苷诱导Bel7402细胞凋亡及其作用机制  

STUDY ON THE APOPTOSIS OF HUMAN HEPATOCELLULAR CARCINOMA CELL LINE BEL7402 CELLS INDUCED BY SOLAMUM LYRATUM THUNB TOTALGLYCOSIDES AND ITS MECHANISM

作　　者：张杰[1] 徐晓宇[2] 张春军[1] 杨旭东[1] 

机构地区：[1]牡丹江医学院,黑龙江牡丹江157011 [2]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150076

出　　处：《牡丹江医学院学报》2011年第2期4-6,共3页Journal of Mudanjiang Medical University

基　　金：黑龙江省卫生厅科研课题(2007-003)

摘　　要：目的:探讨Bc l-2、Fas基因在白毛藤总苷诱导人肝癌Bel7402细胞凋亡中的作用。方法:荧光显微镜观察不同浓度白毛藤总苷诱导Bel7402细胞凋亡作用情况,RT-PCR检测Bcl-2、Fas基因表达量的变化。结果:白毛藤总苷诱导Bel7402细胞凋亡,并且上调Fas和降低Bcl-2表达。结论:白毛藤总苷能促进Bel7402细胞凋亡,其机制可能与激活Fas、抑制Bcl-2表达有关。Objective：To investigate the effect of Bcl-2 and Fas gene in Solamum lyratum Thunb total glycosides inducing apoptosis of Human Hepatocellular carcinoma cell line Bel7402 and the molecular mechanism.Methods：Bel7402 cells were treated with various Solamum lyratum Thunb total glycosides,We observed cell apoptosis by fluorescence microscope.We detected the levels of gene expression in all groups by RT-PCR.Results：Solamum lyratum Thunb total glycosides have obviously apoptosis-inducing effects on Bel7402 cells and could effectively decrease the level of Bcl-2 gene expression and improve the level of Fas gene expression.Conclusion：Solamum lyratum Thunb total glycosides could induce apoptosis of Bel7402 cells probably through down regulating Bcl-2 gene expression and up regulating Fas gene expression.

关 键 词：白毛藤 肝癌细胞 BCL-2基因 FAS基因 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]