大豆株高QTL定位及Meta分析  

Mapping quantitative trait loci and Meta-analysis for plant height in soybean(Glycine max L.Merr.)

作　　者：汪霞[1] 徐宇[1] 李广军[1,2] 李河南[1] 章元明[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室/国家大豆改良中心,江苏南京210095 [2]临沂师范学院,山东临沂276005

出　　处：《南京农业大学学报》2011年第3期13-19,共7页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(30971848);江苏省自然科学基金项目(BK2008335);教育部新世纪优秀人才支持计划项目(NCET-05-0489);教育部111计划项目(B08025);中央高校基本科研业务费专项资助项目(KYT201002)

摘　　要：以溧水中子黄豆和南农493-1杂交衍生的504个正反交F2:4家系为研究对象,于2008年在江苏南京和山东临沂两地种植,鉴定其株高表型。采用多QTL联合分析方法进行QTL分析,检测到株高存在环境效应和细胞质效应,定位了15个主效QTL、2个与环境互作的QTL和6个与细胞质互作的QTL。将Soybase数据库中信息完全的90个株高QTL和本研究检测的株高QTL映射到大豆公共图谱soymap 2上,利用BioMercator 2.1软件进行Meta分析。结果表明:分布于C2、F、L和M染色体上的18个较小置信区间存在一致性QTL,其中包括本研究发现的C2染色体上的qPH-6-2、qPH-6-3和M染色体上的qPH-7-1、qPH-7-2、qPH-7-3共5个QTL。The plant height for 504 F2：4 families derived from direct and reciprocal crosses between Lishuizhongzihuangdou and Nannong 493-1 were measured at Jiangpu experimental station of Nanjing Agricultural University and Linyi experimental station in 2008,respectively.Multi-QTL joint analysis was adopted to detect QTL for the trait.The results showed that environmental and cytoplasmic effects were important for soybean plant height,and fifteen main-effect QTL,two environmental interactions and six cytoplasmic interactions were detected.The above QTL and ninety complete information QTL from Soybase data-base were collected to carry out Meta analysis using the software of BioMercator 2.1.Eighteen consensus QTL were identified,including qPH-6-2 and qPH-6-3 on chromosome C2,and qPH-7-1,qPH-7-2 and qPH-7-3 on chromosome M in this study.

关 键 词：大豆 株高 数量性状基因座 多QTL联合分析 META分析 

分 类 号：S565.1[农业科学—作物学] S332.2