高体积分数氧致慢性肺疾病新生鼠肺泡Ⅱ型上皮细胞超微结构的改变  

Ultrastructure Changes of Alveolar Epithelia Type Ⅱ Cells in Newborn Rats with Chronic Lung Disease Induced by Hyperoxia

作　　者：岳冬梅[1] 佟雅洁[1] 薛辛东[1] 

机构地区：[1]中国医科大学附属盛京医院儿科,沈阳110004

出　　处：《实用儿科临床杂志》2011年第9期691-693,共3页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金(30672253)

摘　　要：目的探讨高体积分数氧(高氧)损伤状态下,新生鼠肺组织通透性及肺泡Ⅱ型上皮细胞(AECⅡ)超微结构的动态变化。方法新生大鼠320只,依据吸氧体积分数(FiO2)分组:实验组1(FiO2800 mL·L-1)、实验组2(FiO2600 mL·L-1)、实验组3(FiO2400 mL·L-1)、空气对照组(FiO2210 mL·L-1)。每组分别于实验1 d、3 d、5 d、7 d、14 d取8只鼠,计算其肺湿/干质量比值(W/D);另取8只新生鼠行支气管肺泡灌洗,检测其支气管肺泡灌洗液(BALF)中蛋白水平的动态变化;透射电镜及硝酸镧示踪技术观察其AECⅡ超微结构及细胞膜通透性改变。结果各实验组5 d、7 d、14 d肺W/D比值明显高于空气对照组,差异均有统计学意义(Pa<0.05),高氧3 d后实验组1与实验组2、实验组3的BALF中蛋白水平与空气对照组比较均显著增高(Pa<0.05);各实验组7 d内随暴露时间延长呈上升趋势;BALF中蛋白水平在实验组3、实验组2、实验组1也呈逐渐增高趋势,且与空气对照组比较均显著增高(Pa<0.05)。实验组AECⅡ的超微结构在出生后不同时间可以出现明显改变,且对硝酸镧颗粒通透性明显增加。结论高氧肺损伤早期存在肺泡上皮通透性增加导致的肺水肿改变;新生鼠随着高氧暴露浓度的增高和时间的延长,肺损伤加重。Objective To investigate the epithelial permeability in lung tissue and the ultrastructure dynamic changes of alveolar epithelia type Ⅱ cells（AEC Ⅱ） under hyperoxia-induced lung injury condition.Methods Three hundred and twenty cases of newborn rats were divided into 4 groups according to different oxygen concentrations（FiO2）： experimental group 1（FiO2 800 mL·L-1）,experimental group 2（FiO2 600 mL·L-1）,experimental group 3（FiO2 400 mL·L-1） and room-air control group（FiO2 210 mL·L-1）.Rats were killed on the 1st,3rd,5th,7th and 14th day after the onset of the experiment.The ultrastructures dynamic changes of AEC Ⅱ and alveolar barrier function through lanthanum nitrate tracing technology were observed.Lung wet/dry weight ratio（W/D） and protein content in bronchoalveolar lavage fluid（BALF） were examined.Results The lung W/D ratios in experimental groups were significantly higher than those in room-air control group on the 5th,7th and 14th day of hyperoxia exposure（Pa0.05）.The protein contents of BALF in experimental groups were significantly higher than those in room-air control group（Pa0.05） and increased as the oxygen concentration elevated and exposure time prolonged.The ultrastructures of AEC Ⅱ changed dramatically with longer exposure duration and the permeability of lanthanum nitrate in experimental groups were much higher than those in room-air control group.Conclusions Increased epithelial permeability which occurred at the early stage of hyperoxia-induced lung injury can lead to pulmonary edema;the lung injury became worse with higher oxygen concentration and longer exposure duration.

关 键 词：高体积分数氧 上皮细胞 肺水肿 大鼠 新生 

分 类 号：R563[医药卫生—呼吸系统]