HPLC同时测定决明子中4种主要成分的含量  

Simultaneous determination of 4 major components in semen cassiae obtusifoline by HPLC

作　　者：苏会娟[1] 王祝举[1] 唐力英[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2011年第10期1327-1329,共3页China Journal of Chinese Materia Medica

摘　　要：目的：建立HPLC同时测定决明子药材中蒽醌类和萘并吡喃酮类4种主要成分含量的方法,为决明子药材的质量控制提供依据。方法：采用Kromasil 100-5 C18色谱柱（4.6 mm×250 mm,5μm）,流动相水-乙腈-四氢呋喃-冰醋酸（100∶23∶5∶1）等度洗脱。检测波长278 nm,柱温30℃,流速1.0 mL.min-1。结果：4种成分达到了基线分离。红链霉素龙胆二糖苷,决明子苷,aurantio-obtusin-6-O-β-D-glucopyranoside以及决明子苷B线性范围为0.2741.37,0.1530.765,0.3021.51,0.0520.26μg,相关系数r均为0.999 9。平均加样回收率分别为100.9%（RSD 1.8%）,101.2%（RSD 1.2%）,99.40%（RSD 2.2%）,100.5%（RSD 1.6%）。结论：建立了高效液相测定决明子中4种主要成分含量的方法,该方法精确,简便,可靠,重现性好,可用于决明子药材的质量控制。Objective： To establish a HPLC method for the simultaneous determination of 4 major components in semen cassiae obtusifoline and provide valuable data for quality control of semen cassiae obtusifoline.Method： Kromasil 100-5 C18column（4.6 mm×250 mm,5 μm）.Eluted with water： acetonitrile： tetramethylene oxide： glacial acetic acid（100∶23∶5∶1）.Detecte at 278 nm.The flow rate was 1.0 mL·min-1.Result： Four compositions were separated well.Good linearies were obtained within the range of 0.274-1.37 μg,0.153-0.765 μg,0.302-1.51 μg and 0.052-0.26 μg for rubrofusarin gentiobioside,casside,aurantio-obtusin-6-O-β-D-glucopyranoside,casside B respectively.The average recoveries were 100.9%（RSD 1.8%）,101.2%（RSD 1.2%）,99.40%（RSD 2.2%）,100.5%（RSD 1.6%）.Conclusion： The method is accurate,reliable and convenient.It can be used to control the quality of semen cassiae obtusifoline and its products.

关 键 词：高效液相色谱法 决明子 红链霉素龙胆二糖苷 aurantio—obtusin-6-O-β-D—glucopyranoside 决明子苷 决明子苷 B 含量测定 

分 类 号：R284[医药卫生—中药学]