中药复方海洋岩盘深层水制剂对顺铂增效减毒作用的研究  

Study on synergic and decreasing toxic effects of mineral water and Chinese herbal compound preparation on cisplatin

作　　者：刘铁铮[1] 李霞[1] 姜青梅[2] 朱慧文[1] 王桂春[1] 

机构地区：[1]山东大学威海分校海洋学院,山东威海264200 [2]威海市立医院肿瘤分院病理科,山东威海264200

出　　处：《中国中药杂志》2011年第10期1358-1361,共4页China Journal of Chinese Materia Medica

基　　金：山东大学威海分校学生科研立项专项基金项目(A09026)

摘　　要：目的:研究中药复方海洋岩盘深层水制剂(MWCHCP)对化疗药物顺铂(cisplatin)的抗肿瘤增效减毒作用。方法:S180荷瘤小鼠腹腔注射顺铂,同时灌胃给予低、中、高剂量MWCHCP(1 925,3 850,7 700 mg.kg-1),连续5 d后,处死小鼠,观察胃组织病理切片,测定肿瘤抑制率、内脏器官指数、腹泻指数,用逆转录-聚合酶链反应(RT-PCR)的方法测定小肠中肿瘤坏死因子-alpha(TNF-α)基因的表达水平。结果:MWCHCP具有协同顺铂抑制肿瘤生长和减轻顺铂所致肝脏、脾脏、肾脏损伤的趋势,可显著减轻顺铂所致小鼠胃损伤并降低腹泻指数,并有降低化疗小鼠小肠组织中TNF-α的表达水平的趋势。结论:MWCHCP对顺铂治疗小鼠S180肉瘤有增效的趋势和显著的减毒作用。Objective： The synergic and decreasing toxic effects of mineral water and Chinese herbal compound preparation（MWCHCP） on cisplatin were investigated in sarcoma 180（S180） mice.Method： The S180 mice were treated for 5 days with intraperitoneal injection of cisplatin（7.33 mg·kg-1） and oral administration of MWCHCP（1 925,3 850,7 700 mg·kg-1）.Then the mice were killed and the tumor growth inhibition rate,organ index,diarrhea index were determined.Observe pathological sections of stomach to study the protective effect of MWCHCP.Reverse transcription-polymerase chain reaction（RT-PCR） was applied to investigate the tumour necrosis factor-alpha（TNF-α） expression level of the intestine.Result： Combining with cisplatin and MWCHCP caused a tendency of increasing the tumor growth inhibition rate and significant attenution of cisplatin-induced diarrhea,visceral organ injury,gastric mucosal injury and decreased TNF-α mRNA level of intestine.Conclusion： The present findings suggest that MWCHCP increases the inhibition rate of tumor growth of cisplatin and has a beneficial influence on gastrointestinal lesion induced by cisplatin.

关 键 词：中药复方海洋岩盘深层水制剂 顺铂 增效减毒 S180 TNF—α 

分 类 号：R285[医药卫生—中药学]