转Bt基因玉米种植对土壤细菌数量及多样性的影响  

Effects of Transgenic Bt Maize on Populations and Diversity of Soil Bacteria

作　　者：刘玲[1,2] 杨殿林[2] 王生荣[1] 赵建宁[2] 李刚[2] 娜布其[2,3] 娜日苏[2,3] 

机构地区：[1]甘肃农业大学草业学院/草业生态系统教育部重点实验室/中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]农业部环境保护科研监测所/农业部转基因生物生态环境安全监督检验测试中心,天津300191 [3]内蒙古师范大学生命科学与技术学院,内蒙古呼和浩特010022

出　　处：《生态与农村环境学报》2011年第3期42-47,共6页Journal of Ecology and Rural Environment

基　　金：转基因生物新品种培育重大专项(2008ZX08012-004);中央级公益性科研院所基本科研业务费专项资金(农业部环境保护科研监测所)

摘　　要：利用三室根箱装置获得玉米生长室土壤(SⅠ)、根际土(SⅡ)、非根际土(SⅢ)3个不同根区土壤,采用传统平板计数培养与变性梯度凝胶电泳(DGGE)技术相结合的方法,研究了转基因玉米B t11及其非转基因亲本在播种后40、50、60 d各根区土壤细菌数量及多样性的变化。结果表明,转B t基因玉米播种后50、60 d SⅠ根区土壤可培养细菌数量显著低于非转基因亲本,播种后40、50 d SⅡ根区土壤可培养细菌数量较亲本玉米显著增加,而在其他时期和根区与亲本玉米之间均无显著差异。DGGE图谱显示,3个采样时期各根区DGGE图谱条带数、土壤细菌多样性指数和均匀度指数均无显著差异(P>0.05)。In this study,the three-compartment rhizobox method was used to collect soil samples from three different root zones of a maize plant,i.e.growth room（SⅠ）,rhizosphere（SⅡ）and non-rhizosphere（SⅢ） and the traditional plate count method and DGGE were applied to investigate changes in population and diversity of soil bacteria at D40,D50 and D60 after sowing of transgenic Bt maize and its parental maize.Results show that cultivation of transgenic Bt maize significantly reduced the population of soil cultivable bacteria in Zone SⅠ at D50 and D60,but increased the population in Zone SⅡat D40 and D50,and showed no significant impact in the other zone.DGGE fingerprints indicated no significant difference between soil samples taken at different dates in DGGE bands,diversity index and homogeneous degree index of the bacteria（P0.05）.

关 键 词：转Bt(Bacillus thuringiensis)玉米 土壤细菌 DGGE 根箱 

分 类 号：S154[农业科学—土壤学]