LuxS基因缺陷对变形链球菌生物膜形成的影响  

The effect of Streptococcus mutans biofilm formation after LuxS was knocked out

作　　者：李月恒[1] 周智[1] 陈娇[1] 尹一兵[2] 张雪梅[2] 

机构地区：[1]重庆医科大学口腔医学院,重庆400015 [2]重庆医科大学检验系,重庆400015

出　　处：《中国微生态学杂志》2011年第5期432-434,共3页Chinese Journal of Microecology

基　　金：重庆市自然科学基金(CSTC2007BB5272);重庆市卫生局科研课题(2008-2-249);重庆市卫生局中医药科研计划项目(2010-2-64)

摘　　要：目的观察LuxS基因缺失后变形链球菌生物膜成熟初期的变化情况。方法通过扫描电镜观察标准菌和缺陷菌在不同营养环境中生物膜成熟初期的形成情况。结果对不同营养环境中形成的生物膜观察,发现在富含蔗糖的环境中,缺陷菌成熟初期的生物膜形成能力较标准菌弱。结论 LuxS基因缺失后变形链球菌在蔗糖环境中生物膜形成的能力减弱。Objective To observe biofilm formation of Streptococcus mutans after its LuxS gene was knocked out under various conditions.Method The strains were cultured in three different conditions（BHI,2% glucose-BHI,2% saccharobiose-BHI）,and the ability of S.mutans and LuxS mutant to form biofilm was examined in 24 h by scanning electron microscopy（SEM）.Result Compared with S.mutans,the LuxS mutant maintained in 2% sucrose displayed an apparent defect in biofilm formation.Conclusion The ability of biofilm formation of Streptococcus mutans will decrease after its LuxS was knocked out while the surroundings are full of sucrose.

关 键 词：变形链球菌 缺陷菌 生物膜 密度感应 

分 类 号：R378.12[医药卫生—病原生物学]