口腔念珠菌基因多态性检测  

Detection of ITS diversity of Candida in oral mucosa

作　　者：郑剑玲[1] 齐贺[1] 张中林[1] 王美慧[1] 耿薇[1] 张伟[1] 

机构地区：[1]辽宁省基础医学研究所免疫微生物教研室,辽宁沈阳110101

出　　处：《中国微生态学杂志》2011年第5期435-437,共3页Chinese Journal of Microecology

摘　　要：目的检测正常人群口腔念珠菌基因型,以了解口腔念珠菌基因多态性。方法采集162例正常人群口腔样本,用念珠菌显色培养基(CHROM agar)进行菌种分离培养鉴定,用玉米Tween80培养基进行真菌孢子形态学检查鉴定,随机抽取45个菌株样本进行内含子转录间隔区(internal transcribed spacer region,ITS)序列检测,并比对同源性,建立进化树,观察菌株进化分支情况。结果分离培养出念珠菌菌株57株(57/162,35.2%),其中白色念珠菌(Candida albicans)36株(36/57,63.1%)。进行ITS序列检测的45个菌株申请GenBank序列注册号为FJ697166-GQ280292。结论正常人群口腔念珠菌基因具有多态性。Objetive To study the Fungi gene diversity by detecting the genotypes of Candida in normal oral mucosa.Method A total of 162 samples from common crowd′s oral mucosa were collected and identified by corn Tween80.CHROM agar was used for isolation,culture and identification.45 strains were randomly selected for ITS sequence detection,homology comparison and drawing the phylogenetic tree.And the strain evolution branch was observed.Result Totally 57 Fungi strains were isolated among 162 samples（57/162,35.2%）,including 36 strains of Candida albicans（36/57,63.1%）.The ITS sequences of the 45 strains were submitted to GenBank,and got the GenBank Accession No.of FJ697166-GQ280292.Conclusion Oral mucosa candidiasis in normal population have genetic polymorphism.

关 键 词：念珠菌 基因多态性 内含子转录间隔区 

分 类 号：R379.4[医药卫生—病原生物学]